TY - JOUR
T1 - Non-universal decoding of the leucine codon CUG in several Candida species
AU - Ohama, Takeshi
AU - Suzuki, Tsutomu
AU - Mori, Miki
AU - Osawa, Syozo
AU - Ueda, Takuya
AU - Watanabe, Kimitsuna
AU - Nakase, Takashi
N1 - Funding Information:
We thank Dr T.H.Jukes (University of California, Berkeley) for discussion and to Drs M.Homma and K.Tanaka (School of Medicine, Nagoya University) for the strain of C.albicans strain C9. This work was supported by a Grant-in-Aid for Scientific Research on Priority Areas from Ministry of Education, Science and Culture of Japan.
PY - 1993/8/25
Y1 - 1993/8/25
N2 - It has been reported that CUG, a universal leucine codon, is read as serine In an asporogenlc yeast, Candida cyllndracea. The distribution of this non-universal genetic code in various yeast species was studied using an In vitro translation assay system with a synthetic messenger RNA containing CUG codons in-frame. It was found that CUG is used as a serine codon in six out of the fourteen species examined, while it is used for leucine in the remaining eight. The tRNA species responsible for the translation of codon CUG as serine was detected in all the six species in which CUG is translated as serine. The grouping according to the CUG codon assignments in these yeast species shows a good correlation with physiological classification by the chain lengths of the Isoprenold moiety of ublquinone and the cell-wall sugar contained in the yeasts. The six Candida species examined in which CUG is used as serine belong to one distinct group in Hemlascomycetes.
AB - It has been reported that CUG, a universal leucine codon, is read as serine In an asporogenlc yeast, Candida cyllndracea. The distribution of this non-universal genetic code in various yeast species was studied using an In vitro translation assay system with a synthetic messenger RNA containing CUG codons in-frame. It was found that CUG is used as a serine codon in six out of the fourteen species examined, while it is used for leucine in the remaining eight. The tRNA species responsible for the translation of codon CUG as serine was detected in all the six species in which CUG is translated as serine. The grouping according to the CUG codon assignments in these yeast species shows a good correlation with physiological classification by the chain lengths of the Isoprenold moiety of ublquinone and the cell-wall sugar contained in the yeasts. The six Candida species examined in which CUG is used as serine belong to one distinct group in Hemlascomycetes.
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U2 - 10.1093/nar/21.17.4039
DO - 10.1093/nar/21.17.4039
M3 - Article
C2 - 8371978
AN - SCOPUS:0027260944
SN - 0305-1048
VL - 21
SP - 4039
EP - 4045
JO - Nucleic acids research
JF - Nucleic acids research
IS - 17
ER -