TY - GEN
T1 - On-chip integration of solid-phase-extraction and silicon pillar arrays for high efficient liquid chromatography
AU - Nakanishi, K.
AU - Shih, K.
AU - Kanamori, T.
AU - Yoon, D. H.
AU - Funatsu, T.
AU - Tsunoda, M.
AU - Sekiguchi, T.
AU - Shoji, S.
N1 - Funding Information:
This work was supported by Japan Ministry of Education, Culture, Sports Science & Technology (MEXT) Grant-in-Aid for Scientific Basic Research (S) No. 23226010 and Scientific Research (C) No. 26460033, and the authors thank for MEXT Nanotechnology Platform Support Project of Waseda University.
Publisher Copyright:
© 15CBMS-0001.
PY - 2015
Y1 - 2015
N2 - Silicon-based solid-phase-extraction (SPE) integrated liquid chromatography (LC) was proposed in this research. The integrated chip allowed continuous operation of selective sample enrichment and chromatography by a simple flow switching process. The sample reservoir was filled with extraction beads after silicon chip fabrication, and then targets were selectively adsorbed onto the beads. Pillar arrays provided uniform and dense sample band, while the targets were released from the beads. The integrated chip achieved more than 160 times higher fluorescence intensity of samples compared to normal injection chips, and coumarin 525 and 545 were successfully separated in 30-mm-long LC channel within 1 min.
AB - Silicon-based solid-phase-extraction (SPE) integrated liquid chromatography (LC) was proposed in this research. The integrated chip allowed continuous operation of selective sample enrichment and chromatography by a simple flow switching process. The sample reservoir was filled with extraction beads after silicon chip fabrication, and then targets were selectively adsorbed onto the beads. Pillar arrays provided uniform and dense sample band, while the targets were released from the beads. The integrated chip achieved more than 160 times higher fluorescence intensity of samples compared to normal injection chips, and coumarin 525 and 545 were successfully separated in 30-mm-long LC channel within 1 min.
KW - Liquid chromatography
KW - On-chip integration
KW - Silicon
KW - Solid phase extraction
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M3 - Conference contribution
AN - SCOPUS:84983371850
T3 - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1936
EP - 1938
BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Y2 - 25 October 2015 through 29 October 2015
ER -