One-step separation of CD20+ cells from whole blood using bacterial magnetic particles displaying protein G

Masayuki Takahashi*, Tomoko Yoshino, Haruko Takeyama, Tadashi Matsunaga

*この研究の対応する著者

研究成果: Conference contribution

抄録

Magnetic separation of target cells from mixtures, such as peripheral blood and bone marrow, has considerable practical potential in research and medical applications. Among the current cell separation techniques, magnetic cell separation using immunomagnetic particles has been routinely applied and has proven rapidness and simplicity. Magnetospirillum magneiicum AMB-1 synthesizes intracellular nano-sized bacterial magnetic particles (BacMPs) that are individually enveloped by a stable lipid bilayer membrane. BacMPs, which exhibit strong ferrimagnetism, can be collected easily with commercially available permanent magnets. In this study, a novel magnetic nanoparticle displaying protein G (protein G-BacMPs) was fabricated, and one-step cell separation for direct cell separation from whole blood was performed using the protein G-BacMPs. B lymphocytes (CD20+ cells), which cover less than 0.3 × 10-2 % of whole blood cells, were separated with 93% purity using protein G-BacMPs binding with anti-CD20 monoclonal antibodies. The results of this study demonstrate the utility of protein G-BacMPs and the magnetic cell separation approach based on protein G-BacMPs in numerous applications.

本文言語English
ホスト出版物のタイトルFrom Biological Materials to Biomimetic Material Synthesis
出版社Materials Research Society
ページ98-103
ページ数6
ISBN(印刷版)9781605608488
DOI
出版ステータスPublished - 2008
外部発表はい

出版物シリーズ

名前Materials Research Society Symposium Proceedings
1094
ISSN(印刷版)0272-9172

ASJC Scopus subject areas

  • 材料科学一般
  • 凝縮系物理学
  • 材料力学
  • 機械工学

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