One-trial conditioning of aerial respiratory behaviour in Lymnaea stagnalis

Kara Martens, Martin Amarell, Kashif Parvez, Karla Hittel, Pascaline De Caigny, Etsuro Ito, Ken Lukowiak*


研究成果: Article査読

33 被引用数 (Scopus)


Repeated spaced training sessions of contingent tactile stimulation to the pneumostome as it opens are required to cause long-term memory (LTM) formation of aerial respiratory behaviour making if difficult to determine exactly when memory forms. We have devised a single-trial aversive operant conditioning training procedure in Lymnaea to be better able to elucidate the causal mechanisms of LTM formation. Observations of baseline breathing behaviour in hypoxia were first made. Twenty-four hours later the snails were trained using the single trial procedure, by placing them in a small Petri dish containing 4 ml of 25 mM KCl for 30-35 s as soon as the first pneumostome opening in hypoxia was attempted. LTM was present if (1) breathing behaviour following training was significantly less than before; and (2) breathing behaviour post-training was significantly less in experimental groups than in yoked control groups. LTM persisted for 24 h but not 48 h. Yoked controls that received an aversive stimulus not contingent with pneumostome opening had no evidence of memory. Cooling directly after, but not at any other time, blocks LTM formation. LTM formation was also prevented by removal of the cell body of the neuron RPeD1 before training.

ジャーナルNeurobiology of Learning and Memory
出版ステータスPublished - 2007 9月

ASJC Scopus subject areas

  • 実験心理学および認知心理学
  • 認知神経科学
  • 行動神経科学


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