TY - JOUR
T1 - PEGylated TiO2 nanoparticles mediated inhibition of cell migration via integrin beta 1
AU - Sun, Qingqing
AU - Kanehira, Koki
AU - Taniguchi, Akiyoshi
N1 - Funding Information:
This work was supported by the National Institute for Materials Science.
Publisher Copyright:
© 2018 The Author(s). Published by National Institute for Materials Science in partnership with Taylor & Francis.
PY - 2018/12/31
Y1 - 2018/12/31
N2 - Nanoparticles (NPs) elicit various physiological responses in cellular environment, and the effect of NPs on cell migration is of high interest. In this work, the effects of NPs on cell migration and their possible mechanisms were studied. Here, we showed that after exposure to pegylated titanium dioxide nanoparticles (TiO2-PEG NPs, where PEG stands for the polyethylene glycol), NCI-H292 cells exhibited slower migration than control cells. Furthermore, larger NPs inhibited cell migration much stronger than smaller NPs. Following NP exposure, the cells showed decreased expression of integrin beta 1 and phosphorylated focal adhesion kinase (pFAK), and disrupted F-actin structures. We demonstrated that a possible mechanism involved NP-mediated promotion of the lysosomal degradation of integrin beta 1, thus leading to reduced expression of pFAK and cytoskeletal disruption and inhibited cell migration. Therefore, our results showed that inhibition of NCI-H292 cell migration by NPs is mediated through integrin beta 1, which provides useful information for the application of NPs in cancer therapy and related fields.
AB - Nanoparticles (NPs) elicit various physiological responses in cellular environment, and the effect of NPs on cell migration is of high interest. In this work, the effects of NPs on cell migration and their possible mechanisms were studied. Here, we showed that after exposure to pegylated titanium dioxide nanoparticles (TiO2-PEG NPs, where PEG stands for the polyethylene glycol), NCI-H292 cells exhibited slower migration than control cells. Furthermore, larger NPs inhibited cell migration much stronger than smaller NPs. Following NP exposure, the cells showed decreased expression of integrin beta 1 and phosphorylated focal adhesion kinase (pFAK), and disrupted F-actin structures. We demonstrated that a possible mechanism involved NP-mediated promotion of the lysosomal degradation of integrin beta 1, thus leading to reduced expression of pFAK and cytoskeletal disruption and inhibited cell migration. Therefore, our results showed that inhibition of NCI-H292 cell migration by NPs is mediated through integrin beta 1, which provides useful information for the application of NPs in cancer therapy and related fields.
KW - Cell migration
KW - TiO-PEG NPs
KW - inhibition
KW - integrin beta 1
KW - pFAK
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U2 - 10.1080/14686996.2018.1444318
DO - 10.1080/14686996.2018.1444318
M3 - Article
AN - SCOPUS:85043587908
SN - 1468-6996
VL - 19
SP - 271
EP - 281
JO - Science and Technology of Advanced Materials
JF - Science and Technology of Advanced Materials
IS - 1
ER -