@article{6bf0cf0c8b064ca1a061c30402fddaa7,
title = "Persistent colonization of non-lymphoid tissue-resident macrophages by Stenotrophomonas maltophilia",
abstract = "Accumulating evidence has revealed that lymphoid tissue-resident commensal bacteria (e.g. Alcaligenes spp.) survive within dendritic cells. We extended our previous study by investigating microbes that persistently colonize colonic macrophages. 16S rRNA-based metagenome analysis using DNA purified from murine colonic macrophages revealed the presence of Stenotrophomonas maltophilia. The in situ intracellular colonization by S. maltophilia was recapitulated in vitro by using bone marrow-derived macrophages (BMDMs). Co-culture of BMDMs with clinically isolated S. maltophilia led to increased mitochondrial respiration and robust IL-10 production. We further identified a 25-kDa protein encoded by the gene assigned as smlt2713 (recently renamed as SMLT-RS12935) and secreted by S. maltophilia as the factor responsible for enhanced IL-10 production by BMDMs. IL-10 production is critical for maintenance of the symbiotic condition, because intracellular colonization by S. maltophilia was impaired in IL-10-deficient BMDMs, and smlt2713-deficient S. maltophilia failed to persistently colonize IL-10-competent BMDMs. These findings indicate a novel commensal network between colonic macrophages and S. maltophilia that is mediated by IL-10 and smlt2713.",
keywords = "IL-10, colitis, gut-resident macrophage, intracellular commensal bacteria, symbiotic factor smlt2713",
author = "Ichiro Takahashi and Koji Hosomi and Takahiro Nagatake and Hirokazu Toubou and Daiki Yamamoto and Ikue Hayashi and Yosuke Kurashima and Shintaro Sato and Naoko Shibata and Yoshiyuki Goto and Fumito Maruyama and Ichiro Nakagawa and Asaomi Kuwae and Akio Abe and Jun Kunisawa and Hiroshi Kiyono",
note = "Funding Information: This research was supported, at least in part, by grants from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT) and the Japan Society for the Promotion of Science (JSPS; kakenhi JP15H05790, JP18H02150, JP18H02674, JP17K09604, JP26670241 and JP26293111 to J.K.; JP19K10088 to I.T.; JP19K07617 to T.N.; and JP18K17997 to K.H.); the Japan Agency for Medical Research and Development (AMED; JP15gm0410004h0106 to I.T.; JP18ek0410032s0103, JP18ak0101068h0002, JP19gm1010006s0103 and 19ek0410062h0001 to J.K.); the grant for Joint Research Project of the Institute of Medical Science, the University of Tokyo (to J.K. and H.K.); Cross-ministerial Strategic Innovation Promotion Program (SIP to J.K.); the Canon Foundation (to J.K.); and the Ministry of Health, Labour, and Welfare of Japan (grant number JP19KA3001 to K.H.). Publisher Copyright: {\textcopyright} 2019 The Author(s) 2019. Published by Oxford University Press on behalf of The Japanese Society for Immunology.",
year = "2019",
month = nov,
day = "12",
doi = "10.1093/intimm/dxz071",
language = "English",
volume = "32",
pages = "133--141",
journal = "International Immunology",
issn = "0953-8178",
publisher = "Oxford University Press",
number = "2",
}