TY - JOUR
T1 - Pestynol, an Antifungal Compound Discovered Using a Saccharomyces cerevisiae 12geneΔ0HSR-iERG6-Based Assay
AU - Sakai, Katsuyuki
AU - Hirose, Tomoyasu
AU - Iwatsuki, Masato
AU - Chinen, Takumi
AU - Kimura, Toru
AU - Suga, Takuya
AU - Nonaka, Kenichi
AU - Nakashima, Takuji
AU - Sunazuka, Toshiaki
AU - Usui, Takeo
AU - Asami, Yukihiro
AU - Omura, Satoshi
AU - Shiomi, Kazuro
N1 - Publisher Copyright:
© 2018 American Chemical Society and American Society of Pharmacognosy.
PY - 2018/7/27
Y1 - 2018/7/27
N2 - The multidrug-sensitive budding yeast, Saccharomyces cerevisiae 12geneΔ0HSR-iERG6, is very useful in antifungal screens. A novel compound, named pestynol (1), was discovered from a culture of the fungus Pestalotiopsis humus FKI-7473 using the multidrug-sensitive yeast. The structure of 1 was elucidated by NMR studies and modified Mosher's method as (1R,2R,3R,4R)-(E)-5-(7,11-dimethyl-3-methylenedodeca-6,10-dien-1-yn-1-yl)cyclohex-5-ene-1,2,3,4-tetraol. Compound 1 showed antimicrobial activity against the Gram-positive bacteria, Klebsiella pneumoniae, and S. cerevisiae 12geneΔ0HSR-iERG6 and Mucor racemosus, but displayed only weak cytotoxicity against various human cancer cell lines. Compound 1 displayed antifungal activities against S. cerevisiae 12geneΔ0HSR-iERG6 and Mucor racemosus at 10 μg/disc.
AB - The multidrug-sensitive budding yeast, Saccharomyces cerevisiae 12geneΔ0HSR-iERG6, is very useful in antifungal screens. A novel compound, named pestynol (1), was discovered from a culture of the fungus Pestalotiopsis humus FKI-7473 using the multidrug-sensitive yeast. The structure of 1 was elucidated by NMR studies and modified Mosher's method as (1R,2R,3R,4R)-(E)-5-(7,11-dimethyl-3-methylenedodeca-6,10-dien-1-yn-1-yl)cyclohex-5-ene-1,2,3,4-tetraol. Compound 1 showed antimicrobial activity against the Gram-positive bacteria, Klebsiella pneumoniae, and S. cerevisiae 12geneΔ0HSR-iERG6 and Mucor racemosus, but displayed only weak cytotoxicity against various human cancer cell lines. Compound 1 displayed antifungal activities against S. cerevisiae 12geneΔ0HSR-iERG6 and Mucor racemosus at 10 μg/disc.
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U2 - 10.1021/acs.jnatprod.8b00200
DO - 10.1021/acs.jnatprod.8b00200
M3 - Article
C2 - 29975062
AN - SCOPUS:85050769896
SN - 0163-3864
VL - 81
SP - 1604
EP - 1609
JO - Journal of Natural Products
JF - Journal of Natural Products
IS - 7
ER -
