TY - JOUR
T1 - Polymer microchip integrated with nano-electrospray tip for electrophoresis-mass spectrometry
AU - Shinohara, Hidetoshi
AU - Suzuki, Takafumi
AU - Kitagawa, Fumihiko
AU - Mizuno, Jun
AU - Otsuka, Koji
AU - Shoji, Shuichi
N1 - Funding Information:
This work is partly supported by a Japan Ministry of Education, Culture, Sports Science & Technology Grant-in-Aid for SCOE of Waseda University “ASMeW,” “The Foundation of Technology Promotion of Electronic Circuit Board,” Scientific Basic Research (C) No. 18560350 and SENTAN. JST. The authors thank the Nanotechnology Support Project of Waseda University for its help.
PY - 2008/6/16
Y1 - 2008/6/16
N2 - A novel cyclo-olefin polymer (COP) microchip was developed integrated with an electrospray ionization (ESI) emitter tip for microchip electrophoretic separation and mass spectrometric detection. The microchip was fabricated by hot embossing and low-temperature direct bonding. The ESI emitter tip structure was formed directly at the microchip electrophoresis (MCE) outlet. Since these microchips enable negligible dead volume at the electrospray port, efficient spray of the sample necessary for acceptable resolution mass spectrometry (MS) was realized. Stable spray was also achieved at the low flow rate (∼0.1 μL/min) without additional pump. By applying the fabricated COP chip to the MCE-ESI-MS analysis, successful separation and detection of caffeine and arginine was achieved.
AB - A novel cyclo-olefin polymer (COP) microchip was developed integrated with an electrospray ionization (ESI) emitter tip for microchip electrophoretic separation and mass spectrometric detection. The microchip was fabricated by hot embossing and low-temperature direct bonding. The ESI emitter tip structure was formed directly at the microchip electrophoresis (MCE) outlet. Since these microchips enable negligible dead volume at the electrospray port, efficient spray of the sample necessary for acceptable resolution mass spectrometry (MS) was realized. Stable spray was also achieved at the low flow rate (∼0.1 μL/min) without additional pump. By applying the fabricated COP chip to the MCE-ESI-MS analysis, successful separation and detection of caffeine and arginine was achieved.
KW - Cyclo-olefin polymer
KW - Electrospray ionization
KW - Mass spectrometry
KW - Microchip electrophoresis
UR - http://www.scopus.com/inward/record.url?scp=44649189102&partnerID=8YFLogxK
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U2 - 10.1016/j.snb.2007.09.071
DO - 10.1016/j.snb.2007.09.071
M3 - Article
AN - SCOPUS:44649189102
SN - 0925-4005
VL - 132
SP - 368
EP - 373
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
IS - 2
ER -