Positive allosteric modulation of the calcium-sensing receptor by physiological concentrations of glucose

Johan Medina, Yuko Nakagawa, Masahiro Nagasawa, Anny Fernandez, Kazushige Sakaguchi, Tetsuya Kitaguchi, Itaru Kojima*


研究成果: Article査読

22 被引用数 (Scopus)


The calcium-sensing receptor (CaSR) is activated by various cations, cationic compounds, and amino acids. In the present study we investigated the effect of glucose on CaSR in HEK293 cells stably expressing human CaSR (HEK-CaSR cells). When glucose concentration in the buffer was raised from 3 to 25 mM, a rapid elevation of cytoplasmic Ca 2+ concentration ([Ca 2+ ]c ) was observed. This elevation was immediate and transient and was followed by a sustained decrease in [Ca 2+ ]c . The effect of glucose was detected at a concentration of 4 mM and reached its maximum at 5 mM. 3-O-Methylglucose, a non-metabolizable analogue of glucose, reproduced the effect of glucose. Sucrose also induced an elevation of [Ca 2+ ]c in HEK-CaSR cells. Similarly, sucralose was nearly as effective as glucose in inducing elevation of [Ca 2+ ]c . Glucose was not able to increase [Ca 2+ ]c in the absence of extracellular Ca 2+ . The effect of glucose on [Ca 2+ ]c was inhibited by NPS-2143, an allosteric inhibitor of CaSR. In addition, NPS-2143 also inhibited the [Ca 2+ ]c responses to sucralose and sucrose. Glucose as well as sucralose decreased cytoplasmic cAMP concentration in HEK-CaSR cells. The reduction of cAMP induced by glucose was blocked by pertussis toxin. Likewise, sucralose reduced [cAMP]c . Finally, glucose increased [Ca 2+ ]c in PT-r parathyroid cells and in Madin-Darby canine kidney cells, both of which express endogenous CaSR. These results indicate that glucose acts as a positive allosteric modulator of CaSR.

ジャーナルJournal of Biological Chemistry
出版ステータスPublished - 2016 10月 28

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 細胞生物学


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