TY - JOUR
T1 - Production of eicosapentaenoic acid by a recombinant marine cyanobacterium, Synechococcus sp
AU - Yu, Reiko
AU - Yamada, Akiko
AU - Watanabe, Kazuo
AU - Yazawa, Kazunaga
AU - Takeyama, Haruko
AU - Matsunaga, Tadashi
AU - Kurane, Ryuichiro
N1 - Funding Information:
This work was performed as a part of The Industrial Science and Technology Project, Technology Development of Biological Resources in Bioconsorcia supported by the New Energy and Industrial Technology Development Organization (NEDO). The authors wish to thank Shizuo Mohara for the GC–MS analysis.
PY - 2000
Y1 - 2000
N2 - The eicosapentaenoic acid (EPA) synthesis gene cluster from an EPA-producing bacterium, Shewanella sp. SCRC-2738, was cloned into a broad-host range vector, pJRD215, and then introduced into a marine cyanobacterium, Synechococcus sp. NKBG15041c, by conjugation. The transconjugant cyanobacteria produced 3.7 ± 0.2% (2.24 ± 0.13 mg/L) EPA (n-3) and 2.5 ± 0.2% (1.49 ± 0.06 mg/L) eicosatetraenoic acid (n-3) of the total fatty acids when the cells were cultured at 23°C at a light intensity of 1,000-1,500 Lux. The EPA and eico-satetraenoic acid contents of the cells were increased to 4.6 ± 0.6% (3.86 ± 1.11 mg/L) and 4.7 ± 0.3% (3.86 ± 0.82 mg/L), and 7.5 ± 0.3% (1.76 ± 0.10 mg/L) and 5.1 ± 0.2% (1.19 ± 0.06 mg/L) when they were cultured at low temperature (18°C) and at lower light intensity (40 Lux), respectively.
AB - The eicosapentaenoic acid (EPA) synthesis gene cluster from an EPA-producing bacterium, Shewanella sp. SCRC-2738, was cloned into a broad-host range vector, pJRD215, and then introduced into a marine cyanobacterium, Synechococcus sp. NKBG15041c, by conjugation. The transconjugant cyanobacteria produced 3.7 ± 0.2% (2.24 ± 0.13 mg/L) EPA (n-3) and 2.5 ± 0.2% (1.49 ± 0.06 mg/L) eicosatetraenoic acid (n-3) of the total fatty acids when the cells were cultured at 23°C at a light intensity of 1,000-1,500 Lux. The EPA and eico-satetraenoic acid contents of the cells were increased to 4.6 ± 0.6% (3.86 ± 1.11 mg/L) and 4.7 ± 0.3% (3.86 ± 0.82 mg/L), and 7.5 ± 0.3% (1.76 ± 0.10 mg/L) and 5.1 ± 0.2% (1.19 ± 0.06 mg/L) when they were cultured at low temperature (18°C) and at lower light intensity (40 Lux), respectively.
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U2 - 10.1007/s11745-000-0619-6
DO - 10.1007/s11745-000-0619-6
M3 - Article
C2 - 11104010
AN - SCOPUS:0033744916
SN - 0024-4201
VL - 35
SP - 1061
EP - 1064
JO - Lipids
JF - Lipids
IS - 10
ER -
