Proliferation of mammalian cells with Chlorococcum littorale algal compounds without serum support

Jayeesha Ghosh, Yoshikatsu Akiyama, Yuji Haraguchi, Kumiko Yamanaka, Toru Asahi, Yoichi Nakao, Tatsuya Shimizu*

*この研究の対応する著者

研究成果: Article査読

2 被引用数 (Scopus)

抄録

In recent years, serum-free medium for mammalian cell cultivation has attracted a lot of attention, considering the high cost of production and environmental load involved in developing the conventional animal sera. The use of alternative growth-promoting products in mammalian cell cultivation such as extracts from microalgae has proven to be quite beneficial and environmental-friendly. This research aims to cultivate mammalian cells with growth-promoting factors derived from Chlorococcum littorale. We have established a simple extraction using the ultrasonication method and applied the extract in place of serum on mammalian C2C12 cell lines, 3T3 cell lines, and CHO cell lines to compare and analyze the effectiveness of the extract. Cell passage was conducted in a suspended culture condition with the addition of the extract. The results indicate that the extract from microalgae shows a high proliferation rate in all cell lines without fetal bovine serum. Moreover, it is eco-friendly and has huge potential to replace the traditional cell culture system. It could be applied in the fields of regenerative medicine, gene/cell therapies, as well as cultured meat production.

本文言語English
論文番号e3402
ジャーナルBiotechnology Progress
40
1
DOI
出版ステータスPublished - 2024 1月 1

ASJC Scopus subject areas

  • バイオテクノロジー

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