抄録
An endoplasmic reticulum (ER) protein, protein disulfide isomerase (PDI), was found to locate in the acrosome of rat spermatids and sperm by the avidin-biotin-peroxidasecomplex (ABC) method. Immunoblot analysis of PDI during developmental stages showed that protein was detected in the testis between the ages of 5 and 50 days. When Northern blot analysis was carried out using human PDI cDNA as a probe, a 2.6-kb PDI mRNA was found in both the spermatocytes and spermatids of rat testis. The PDI mRNA level was similar in both types of cells. It would appear that PDI may stabilize three-dimensional structures of secretory proteins in the acrosome of rat spermatids during spermiogenesis.
| 本文言語 | English |
|---|---|
| ホスト出版物のタイトル | Molecular Andrology |
| ページ | 201-208 |
| ページ数 | 8 |
| 巻 | 5 |
| 版 | 4 |
| 出版ステータス | Published - 1993 |
| 外部発表 | はい |
ASJC Scopus subject areas
- 内分泌学
- 腎臓病学