Proteomic profiling of intestinal epithelial-like cell-derived exosomes regulated by epigallocatechin gallate

Satoshi Yano*, Katsuhiko Suzuki, Taichi Hara*

*この研究の対応する著者

研究成果: Article査読

抄録

Exosomes are extracellular vesicles primarily responsible for intercellular communication, and they contain nucleic acids and proteins. Exosome secretion has been observed in the intestines, suggesting their physiological effects on the receptor cells of target tissues. It is possible that intestinal epithelial cells recognize food components as ligands, resulting in exosome secretion. However, research on intestine-derived exosomes regulated by food ingredients is limited. In this study, Caco-2 cells were utilized as an intestinal epithelial model for proteomic profiling. NanoLC-MS/MS analysis revealed the alteration of exosome properties by epigallocatechin gallate (EGCG) in differentiated Caco-2 cells. This natural polyphenol reduced both the number and size of secreted exosomes and altered the expression of exosomal proteins. The enriched proteins in exosomes were involved in immune response and cell proliferation. In contrast, those in the EGCG-treated group had distinctive functions in the maintenance of skin homeostasis. We also found variable expression of galectin-3-binding protein and fibronectin as molecular signatures in exosomes derived from EGCG-treated cells. These results could help elucidate the expression and mechanism of exosomal proteins related to food components.

本文言語English
ページ(範囲)390-404
ページ数15
ジャーナルBioFactors
49
2
DOI
出版ステータスPublished - 2023 3月 1

ASJC Scopus subject areas

  • 生化学
  • 分子医療
  • 臨床生化学

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