Pyrosequencing analysis of bacterial species affected by ethanol-extract from activated sludge

Tao Su*, Hiroyasu Satoh, Wataru Suda, Masahira Hattori, Takashi Mino


研究成果: Article査読

1 被引用数 (Scopus)


Bacteria play a major role in removal of pollutants in activated sludge processes, but the reason why bacterial population changes is not yet clearly understood. This study focused on the chemicals produced by microorganisms in activated sludge. Chemical components in an activated sludge from a laboratory sequencing batch reactor (Lab SBR) was extracted with ethanol, and activated sludges from two sources, Lab SBR and a wastewater treatment plant (WWTP) were incubated with and without the extracted chemicals. Incubation was performed on a microplate which has 96 wells and each of the well is regarded as a small activated sludge reactor. After 24 hours incubation, activated sludge samples were recovered, and their microbial populations were analyzed by reverse transcription PCR (RT-PCR) targeting a partial 16S rRNA gene sequence followed by pyrosequencing. The results were preliminary, but clearly showed that bacterial population changed significantly when activated sludge was incubated with ethanol-extract addition. In addition, activated sludge from Lab SBR and WWTP were similarly affected by the extract.

ジャーナルJournal of Water and Environment Technology
出版ステータスPublished - 2019 1月 1

ASJC Scopus subject areas

  • 環境工学
  • 生態モデリング
  • 水の科学と技術
  • 廃棄物管理と処理
  • 汚染
  • 健康、毒物学および変異誘発


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