@inproceedings{98dbad1daa8643888ba7804f033b94e7,
title = "Quantitative determination of branched-chain amino acids in human plasma using pressure-driven liquid chromatography with pillar array columns",
abstract = "We have succeeded for the first time to quantify biological compounds in human plasma using pillar array columns. A fast and quantitative method for the determination of branched-chain amino acids (BCAAs) in human plasma using pressure-driven liquid chromatography on a chip was developed. BCAAs were firstly derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), and then analysis of NBD-BCAAs was optimized to be finished in 100 s. With the help of an internal standard, the concentrations of BCAAs in human plasma samples were quantitatively determined. The developed method should be a useful tool for fast diagnosis in clinical field.",
keywords = "Fluorescence, Internal standard, NBD-F",
author = "Y. Song and K. Takatsuki and M. Isokawa and T. Sekiguchi and J. Mizuno and T. Funatsu and S. Shoji and M. Tsunoda",
year = "2013",
month = jan,
day = "1",
language = "English",
isbn = "9781632666246",
series = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
publisher = "Chemical and Biological Microsystems Society",
pages = "1794--1796",
booktitle = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
note = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 ; Conference date: 27-10-2013 Through 31-10-2013",
}