Raman and mid-infrared spectroscopic imaging: Applications and advancements

Using Raman and Mid-Infrared (MIR) spectroscopic imaging techniques one can examine the spatial distribution of various molecular constituents in a heterogeneous sample at a microscopic scale. Raman and MIR spectroscopy techniques provide bond-specific vibrational frequencies to characterize molecular species without external labelling. In addition, these techniques are rapid, non-invasive and provide multiplex advantage. In this review we discuss the instrumentation, applications and latest advancements of Raman and MIR spectroscopic imaging in various fields, ranging from materials science to medicine and illustrate with specific examples from our recent research.