TY - JOUR
T1 - Raman Microspectroscopy Imaging Analysis of Extracellular Vesicles Biogenesis by Filamentous Fungus Penicilium chrysogenum
AU - Samuel, Ashok Zachariah
AU - Horii, Shumpei
AU - Nakashima, Takuji
AU - Shibata, Naoko
AU - Ando, Masahiro
AU - Takeyama, Haruko
N1 - Funding Information:
The authors thank Dr. Hiroshi Sagara and Dr. Yuji Watanabe, Institute of medical science, University of Tokyo for their help in recording TEM images. This work was supported by JSPS KAKENHI Grant No. JP17H06158.
Funding Information:
The authors thank Dr. Hiroshi Sagara and Dr. Yuji Watanabe, Institute of medical science, University of Tokyo for their help in recording TEM images. This work was supported by JSPS KAKENHI Grant No. JP17H06158.
Publisher Copyright:
© 2022 The Authors. Advanced Biology published by Wiley-VCH GmbH.
PY - 2022/6
Y1 - 2022/6
N2 - The mechanism of production of extracellular vesicles (EVs) and their molecular contents are of great interest due to their diverse roles in biological systems and are far from being completely understood. Even though cellular cargo releases mediated by EVs have been demonstrated in several cases, their role in secondary metabolite production and release remains elusive. In this study, this aspect is investigated in detail using Raman microspectroscopic imaging. Considerable evidence is provided to suggest that the release of antibiotic penicillin by the filamentous fungus Penicillium chrysogenum involves EVs. Further, the study also reveals morphological modifications of the fungal body during biogenesis, changes in cell composition at the locus of biogenesis, and major molecular contents of the released EVs. The results suggest a possible general role of EVs in the release of antibiotics from the producing organisms.
AB - The mechanism of production of extracellular vesicles (EVs) and their molecular contents are of great interest due to their diverse roles in biological systems and are far from being completely understood. Even though cellular cargo releases mediated by EVs have been demonstrated in several cases, their role in secondary metabolite production and release remains elusive. In this study, this aspect is investigated in detail using Raman microspectroscopic imaging. Considerable evidence is provided to suggest that the release of antibiotic penicillin by the filamentous fungus Penicillium chrysogenum involves EVs. Further, the study also reveals morphological modifications of the fungal body during biogenesis, changes in cell composition at the locus of biogenesis, and major molecular contents of the released EVs. The results suggest a possible general role of EVs in the release of antibiotics from the producing organisms.
KW - Raman spectroscopy
KW - extracellular vesicles
KW - multivariate curve resolution
KW - penicillin
KW - penicillium chrysogenum
KW - secondary metabolite
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U2 - 10.1002/adbi.202101322
DO - 10.1002/adbi.202101322
M3 - Article
C2 - 35277945
AN - SCOPUS:85126032386
SN - 2701-0198
VL - 6
JO - Advanced Biology
JF - Advanced Biology
IS - 6
M1 - 2101322
ER -