TY - JOUR
T1 - Rapid detection of hemagglutination using restrictive microfluidic channels equipped with waveguide-mode sensors
AU - Ashiba, Hiroki
AU - Fujimaki, Makoto
AU - Awazu, Koichi
AU - Fu, Mengying
AU - Ohki, Yoshimichi
AU - Tanaka, Torahiko
AU - Makishima, Makoto
N1 - Funding Information:
This work was supported by SENTAN, Japan Science and Technology Agency (JST). Part of the microfluidic channel fabrication was conducted at the AIST Nano-Processing Facility, supported by the "Nanotechnology Platform Program" of the Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT). Original wafers of the waveguide-mode sensor chips were supplied by the Advanced Functional Materials Research Center of Shin- Etsu Chemical Co., Ltd.
Publisher Copyright:
© 2016 The Japan Society of Applied Physics.
PY - 2016/2
Y1 - 2016/2
N2 - Hemagglutination is utilized for various immunological assays, including blood typing and virus detection. Herein, we describe a method of rapid hemagglutination detection based on a microfluidic channel installed on an optical waveguide-mode sensor. Human blood samples mixed with hemagglutinating antibodies associated with different blood groups were injected into the microfluidic channel, and reflectance spectra of the samples were measured after stopping the flow. The agglutinated and nonagglutinated samples were distinguishable by the alterations in their reflectance spectra with time; the microfluidic channels worked as spatial restraints for agglutinated red blood cells. The demonstrated system allowed rapid hemagglutination detection within 1 min. The suitable height of the channels was also discussed.
AB - Hemagglutination is utilized for various immunological assays, including blood typing and virus detection. Herein, we describe a method of rapid hemagglutination detection based on a microfluidic channel installed on an optical waveguide-mode sensor. Human blood samples mixed with hemagglutinating antibodies associated with different blood groups were injected into the microfluidic channel, and reflectance spectra of the samples were measured after stopping the flow. The agglutinated and nonagglutinated samples were distinguishable by the alterations in their reflectance spectra with time; the microfluidic channels worked as spatial restraints for agglutinated red blood cells. The demonstrated system allowed rapid hemagglutination detection within 1 min. The suitable height of the channels was also discussed.
UR - http://www.scopus.com/inward/record.url?scp=84961290554&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84961290554&partnerID=8YFLogxK
U2 - 10.7567/JJAP.55.027002
DO - 10.7567/JJAP.55.027002
M3 - Article
AN - SCOPUS:84961290554
SN - 0021-4922
VL - 55
JO - Japanese journal of applied physics
JF - Japanese journal of applied physics
IS - 2
M1 - 027002
ER -