TY - JOUR
T1 - Rat Ovarian 20±-Hydroxysteroid Dehydrogenase-1 May Belong to Aldo-Keto Reductase Superfamily
AU - Noda, Ken
AU - Shiota, Kunio
AU - Ogawa, Tomoya
AU - Yagi, Shintaro
AU - Takahashi, Michio
PY - 1993
Y1 - 1993
N2 - We have previously purified the 20±hydroxysteroid dehydrogenase (20±HSD) molecule from normal mature rat ovarian cytosol [Biochim. Biophys. Acta, 1991, 1079: 112-118]. In order to further characterize the 20±HSD and to obtain information for molecular cloning of the enzyme, we determined its N-terminal amino acid sequence. The sequence was shown to be Ser-Lys-lle-Gln-Lys-Met-Glu-Leu-Asn-Asp-Gly-His-Ser-lle-Pro-Val-Leu-Gly-Phe-Xaa-Thr. A search of the SWISS-PROT protein sequence database for N-terminal amino acid sequence similarities revealed 6 highly homologous proteins, including bovine prostaglandin F synthase, rat liver 3±hydroxysteroid dehydrogenase, bovine lens aldose reductase, human aldose reductase, frog 3±crystallin and human liver chlordecone reductase, suggesting that rat ovarian 20ar-HSD belongs to the aldo-keto reductase gene superfamily.
AB - We have previously purified the 20±hydroxysteroid dehydrogenase (20±HSD) molecule from normal mature rat ovarian cytosol [Biochim. Biophys. Acta, 1991, 1079: 112-118]. In order to further characterize the 20±HSD and to obtain information for molecular cloning of the enzyme, we determined its N-terminal amino acid sequence. The sequence was shown to be Ser-Lys-lle-Gln-Lys-Met-Glu-Leu-Asn-Asp-Gly-His-Ser-lle-Pro-Val-Leu-Gly-Phe-Xaa-Thr. A search of the SWISS-PROT protein sequence database for N-terminal amino acid sequence similarities revealed 6 highly homologous proteins, including bovine prostaglandin F synthase, rat liver 3±hydroxysteroid dehydrogenase, bovine lens aldose reductase, human aldose reductase, frog 3±crystallin and human liver chlordecone reductase, suggesting that rat ovarian 20ar-HSD belongs to the aldo-keto reductase gene superfamily.
KW - 20α-Hydroxy steroid dehydrogenase
KW - Aldo-keto reductase
KW - Automatic Edman degradation
KW - N-terminal amino acid sequence
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U2 - 10.1262/jrd.39.169
DO - 10.1262/jrd.39.169
M3 - Article
AN - SCOPUS:85010089845
SN - 0916-8818
VL - 39
SP - 169
EP - 173
JO - Journal of Reproduction and Development
JF - Journal of Reproduction and Development
IS - 2
ER -