@article{c2b7daadf9da4ce7a359b52edb51153c,
title = "Real-Time Monitoring of Glucose Export from Isolated Chloroplasts Using an Organic Electrochemical Transistor",
abstract = "Biosensors based on organic electrochemical transistors (OECT) are attractive devices for real-time monitoring of biological processes. The direct coupling between the channel of the OECT and the electrolyte enables intimate interfacing with biological environments at the same time bringing signal amplification and fast sensor response times. So far, these devices are mainly applied to mammalian systems; cells or body fluids for the development of diagnostics and various health status monitoring technology. Yet, no direct detection of biomolecules from cells or organelles is reported. Here, an OECT glucose sensor applied to chloroplasts, which are the plant organelles responsible for the light-to-chemical energy conversion of the photosynthesis, is reported. Real-time monitoring of glucose export from chloroplasts in two distinct metabolic phases is demonstrated and the transfer dynamics with a time resolution of 1 min is quantified, thus reaching monitoring dynamics being an order of magnitude better than conventional methods.",
keywords = "chloroplasts, glucose sensing, organic electrochemical transistor, photosynthesis, real-time monitoring",
author = "Chiara Diacci and Lee, {Jee Woong} and Per Janson and Gwenna{\"e}l Dufil and G{\'a}bor M{\'e}hes and Magnus Berggren and Simon, {Daniel T.} and Eleni Stavrinidou",
note = "Funding Information: C.D., J.W.L., and P.J. contributed equally to this work. The authors wish to thank {\AA}sa Strand, Tamara Hern{\'a}ndez-Verdeja, and Totte Niittyl{\"a} from Ume{\aa} Plant Science Center, Benoit Piro from Paris-Diderot University and Xenofon Strakosas from Link{\"o}ping University for fruitful discussions. Funding was provided by the Knut and Alice Wallenberg Foundation, Link{\"o}ping University and industry through the Wallenberg Wood Science Center, the Swedish Foundation for Strategic Research (SSF), Vetenskapsr{\aa}det (VR), {\"O}nnesj{\"o} Foundation, VINNOVA and the Swedish Government Strategic Research Area in Materials Science on Functional Materials at Link{\"o}ping University (Faculty Grant SFO-Mat-LiU No. 2009-00971). Additional funding was provided by the European Union's Horizon 2020 research and innovation programme under Grant Agreement No. 800926 (FET-OPEN-HyPhOE) to E.S. and G.D. and a Marie Sklodowska Curie Individual Fellowship (MSCA-IFEF-ST, Trans-Plant, 702641) to E.S. GM was also supported by a grant from the Swedish MSCA Seal of Excellence program. Publisher Copyright: {\textcopyright} 2019 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",
year = "2020",
month = mar,
day = "1",
doi = "10.1002/admt.201900262",
language = "English",
volume = "5",
journal = "Advanced Materials Technologies",
issn = "2365-709X",
publisher = "Wiley-Blackwell",
number = "3",
}