@article{5d012bde3bff4bf390b814c11279ee8f,
title = "Reconstitution of 30S ribosomal subunits in vitro using ribosome biogenesis factors",
abstract = "Reconstitution of ribosomes in vitro from individual ribosomal proteins provides a powerful tool for understanding the ribosome assembly process including the sequential incorporation of ribosomal proteins. However, conventional assembly methods require high-salt conditions for efficient ribosome assembly. In this study, we reconstituted 30S ribosomal subunits from individually purified ribosomal proteins in the presence of ribosome biogenesis factors. In this system, two GTPases (Era and YjeQ) facilitated assembly of a 30S subunit exhibiting poly(U)-directed polyphenylalanine synthesis and native protein synthesis under physiological conditions. This in vitro system permits a study of the assembly process and function of ribosome biogenesis factors, and it will facilitate the generation of ribosomes from DNA without using cells.",
keywords = "Biogenesis factors, Cell-free translation system, In vitro reconstitution, Ribosome, Synthetic biology",
author = "Daichi Tamaru and Kazuaki Amikura and Yoshihiro Shimizu and Nierhaus, {Knud H.} and Takuya Ueda",
note = "Funding Information: We thank Dr. Takashi Kanamori for providing us a protein and for stimulating discussions in this project; and Mr. Yuki Ohkoshi for pilot experiments of ribosomal protein purification. The founders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. This work was supported in part by the Japan Society for the Promotion of Science (JSPS) KAKENHI (10J06199 to D.T.; 15K16083 to K.A.; 16H02089 to T.U.; 26710014, 26640134, and 17H05680 to Y.S.), the Human Frontier Science Program (RGP0008/2014 to K.H.N. and T.U.), the Astrobiology Center Project of the National Institutes of Natural Sciences (AB271004 and AB281007 to K.A.), Grant for Basic Science Research Projects from the Sumitomo Foundation (140953 to K.A.), the Strategic Programs for R&D (President{\textquoteright}s Discretionary Fund) of RIKEN (to Y.S.), and an intramural Grant-in-Aid from the RIKEN Quantitative Biology Center (to Y.S.). Publisher Copyright: {\textcopyright} 2018 Tamaru et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society",
year = "2018",
month = nov,
doi = "10.1261/rna.065615.118",
language = "English",
volume = "24",
pages = "1512--1519",
journal = "RNA",
issn = "1355-8382",
publisher = "Cold Spring Harbor Laboratory Press",
number = "11",
}