抄録
Submega-sized regions of the Bacillus subtilis genome were cloned to plasmid by the B. subtilis Recombinational Transfer (BReT) method. BReT efficiency depends not only on the genome location but also on the choice of sequences for simultaneous homologous recombination during BReT. In an extreme case, a 91-kb region that was unsuccessful on the first attempt was obtained when the slightly shifted 98-kb region was targeted.
| 本文言語 | English |
|---|---|
| ページ（範囲） | 1382-1384 |
| ページ数 | 3 |
| ジャーナル | Bioscience, Biotechnology and Biochemistry |
| 巻 | 68 |
| 号 | 6 |
| DOI | |
| 出版ステータス | Published - 2004 6月 |
| 外部発表 | はい |
ASJC Scopus subject areas
- 食品科学
- 応用微生物学とバイオテクノロジー
- 化学（その他）
- 生化学、遺伝学、分子生物学（全般）
- 生化学
- バイオテクノロジー