@inproceedings{db545083d5a44fd49845aca9e7bd6b64,
title = "Room temperature uniform and high throughput agarose gel micro droplet generation for single cell analysis",
abstract = "Uniform agarose gel micro droplet (GMD) generation method was developed using simple cross-junction microfluidic device without precise temperature control. Ultralow gelling temperature agarose was used as cell-enclosing capsule material because it remains in liquid phase from 20 °C to 45 °C. About 50 μm in diameter GMD which is suitable size for the target cell culturing media is generated with generation rate of about 200 drops/sec and size deviation smaller than 3.83 %. Encapsulated single E.coli cell was cultured successfully in the agarose GMD for 3 hours. Single cell encapsulated GMD generation ratio was about 31 %.",
keywords = "Cell encapsulation, Gel micro droplet (GMD), Microfluidics, Single cell",
author = "T. Hirose and Y. Hoshino and Yoon, {D. H.} and T. Mori and T. Sekiguchi and H. Takeyama and S. Shoji",
year = "2013",
month = jan,
day = "1",
language = "English",
isbn = "9781632666246",
series = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
publisher = "Chemical and Biological Microsystems Society",
pages = "440--442",
booktitle = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
note = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 ; Conference date: 27-10-2013 Through 31-10-2013",
}