TY - JOUR
T1 - Skewed X-inactivation in cloned mice
AU - Senda, Sho
AU - Wakayama, Teruhiko
AU - Yamazaki, Yukiko
AU - Ohgane, Jun
AU - Hattori, Naka
AU - Tanaka, Satoshi
AU - Yanagimachi, Ryuzo
AU - Shiota, Kunio
PY - 2004/8/13
Y1 - 2004/8/13
N2 - In female mammals, dosage compensation for X-linked genes is accomplished by inactivation of one of two X chromosomes. The X-inactivation ratio (a percentage of the cells with inactivated maternal X chromosomes in the whole cells) is skewed as a consequence of various genetic mutations, and has been observed in a number of X-linked disorders. We previously reported that phenotypically normal full-term cloned mouse fetuses had loci with inappropriate DNA methylation. Thus, cloned mice are excellent models to study abnormal epigenetic events in mammalian development. In the present study, we analyzed X-inactivation ratios in adult female cloned mice (B6C3F1). Kidneys of eight naturally produced controls and 11 cloned mice were analyzed. Although variations in X-inactivation ratio among the mice were observed in both groups, the distributions were significantly different (Ansary-Bradley test, P
AB - In female mammals, dosage compensation for X-linked genes is accomplished by inactivation of one of two X chromosomes. The X-inactivation ratio (a percentage of the cells with inactivated maternal X chromosomes in the whole cells) is skewed as a consequence of various genetic mutations, and has been observed in a number of X-linked disorders. We previously reported that phenotypically normal full-term cloned mouse fetuses had loci with inappropriate DNA methylation. Thus, cloned mice are excellent models to study abnormal epigenetic events in mammalian development. In the present study, we analyzed X-inactivation ratios in adult female cloned mice (B6C3F1). Kidneys of eight naturally produced controls and 11 cloned mice were analyzed. Although variations in X-inactivation ratio among the mice were observed in both groups, the distributions were significantly different (Ansary-Bradley test, P
KW - Cloned mouse
KW - DNA methylation
KW - Epigenetics
KW - Skewed X-inactivation
KW - X-chromosome inactivation
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U2 - 10.1016/j.bbrc.2004.06.096
DO - 10.1016/j.bbrc.2004.06.096
M3 - Article
C2 - 15358212
AN - SCOPUS:3542996285
SN - 0006-291X
VL - 321
SP - 38
EP - 44
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -