TY - GEN
T1 - Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture
AU - Sugio, Yoshihiro
AU - Moriguchi, Hiroyuki
AU - Suzuki, Ikurou
AU - Kaneko, Tomoyuki
AU - Yasuda, Kenji
AU - Jimbo, Yasuhiko
N1 - Publisher Copyright:
© 2003 Japan Soc. of Applied.
PY - 2003
Y1 - 2003
N2 - We have developed a new type of on-chip cell cultivation system using an agarose microchamber (AMC) array and a photo-thermal etching method, thus enabling topographical control of neuronal network pattern step-by-step during cell cultivation. By using photo-thermal etching (micro melting) method, the number of microtunnel connecting microchambers can be easily increased, even during cell cultivation, according to the progress of the neuronal network formation. To demonstrate the capability of this system for topographical control of network formation, we cultured hippocampal neurons in this AMC array. We found that the cells in microchambers made fiber connections through microtunnels. Furthermore the cells even made fiber connections through additional microtunnels fabricated during cultivation by photo-thermal etching. The results showed that the photo-thermal etching could be used during cultivation without damaging cells.
AB - We have developed a new type of on-chip cell cultivation system using an agarose microchamber (AMC) array and a photo-thermal etching method, thus enabling topographical control of neuronal network pattern step-by-step during cell cultivation. By using photo-thermal etching (micro melting) method, the number of microtunnel connecting microchambers can be easily increased, even during cell cultivation, according to the progress of the neuronal network formation. To demonstrate the capability of this system for topographical control of network formation, we cultured hippocampal neurons in this AMC array. We found that the cells in microchambers made fiber connections through microtunnels. Furthermore the cells even made fiber connections through additional microtunnels fabricated during cultivation by photo-thermal etching. The results showed that the photo-thermal etching could be used during cultivation without damaging cells.
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U2 - 10.1109/IMNC.2003.1268776
DO - 10.1109/IMNC.2003.1268776
M3 - Conference contribution
AN - SCOPUS:84949211503
T3 - Digest of Papers - Microprocesses and Nanotechnology 2003 - 2003 International Microprocesses and Nanotechnology Conference, MNC 2003
SP - 322
EP - 323
BT - Digest of Papers - Microprocesses and Nanotechnology 2003 - 2003 International Microprocesses and Nanotechnology Conference, MNC 2003
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - International Microprocesses and Nanotechnology Conference, MNC 2003
Y2 - 29 October 2003 through 31 October 2003
ER -