TY - JOUR
T1 - Stimulation of Spermatid Phosphofructokinase by Fructose 2,6‐Bisphosphate from Rat Testes
T2 - fructose 2, 6‐bisphosphate/phosphofructokinase/spermatids/rats
AU - NAKAMURA, MASAHISA
AU - SUZUKI, AKIRA
AU - OKINAGA, SHOICHI
AU - ARAI, KIYOSHI
PY - 1984
Y1 - 1984
N2 - Effect of fructose 2, 6‐bisphosphate on 6‐phosphofructokinase (ATP: D‐fructose‐6‐phosphate 1‐phosphotransferase, EC 2.7.1.11) in spermatid extract from rat testes was studied. Fructose 2, 6‐bisphosphate stimulated the enzyme greatly by increasing its affinity for fructose 6‐phosphate and relieving the inhibition by ATP. Fructose 2, 6‐bisphosphate (0.8 μM) was required for 50% activation of 6‐phosphofructokinase (PFK). In addition, fructose 2, 6‐bisphosphate, AMP and fructose 6‐phosphate acted cooperatively to stimulate the activity of PFK. This stimulation may play an important role in the regulation of glycolysis in spermatids of rat testes.
AB - Effect of fructose 2, 6‐bisphosphate on 6‐phosphofructokinase (ATP: D‐fructose‐6‐phosphate 1‐phosphotransferase, EC 2.7.1.11) in spermatid extract from rat testes was studied. Fructose 2, 6‐bisphosphate stimulated the enzyme greatly by increasing its affinity for fructose 6‐phosphate and relieving the inhibition by ATP. Fructose 2, 6‐bisphosphate (0.8 μM) was required for 50% activation of 6‐phosphofructokinase (PFK). In addition, fructose 2, 6‐bisphosphate, AMP and fructose 6‐phosphate acted cooperatively to stimulate the activity of PFK. This stimulation may play an important role in the regulation of glycolysis in spermatids of rat testes.
UR - http://www.scopus.com/inward/record.url?scp=84985855349&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84985855349&partnerID=8YFLogxK
U2 - 10.1111/j.1440-169X.1984.00011.x
DO - 10.1111/j.1440-169X.1984.00011.x
M3 - Article
AN - SCOPUS:84985855349
SN - 0012-1592
VL - 26
SP - 11
EP - 16
JO - Development Growth and Differentiation
JF - Development Growth and Differentiation
IS - 1
ER -