TY - JOUR
T1 - Structural polymorphism of the Escherichia coli poly-α-L-glutamate synthetase RimK
AU - Arimura, Yasuhiro
AU - Kono, Tomonori
AU - Kino, Kuniki
AU - Kurumizaka, Hitoshi
N1 - Funding Information:
HK and KK were supported by the Waseda Research Institute for Science and Engineering. YA was supported by the Early Bird Program of the Waseda Research Institute for Science and Engineering.
Publisher Copyright:
© 2018 International Union of Crystallography.
PY - 2018/7
Y1 - 2018/7
N2 - Bacterial RimK is an enzyme that catalyzes the polyglutamylation of the C-terminus of ribosomal protein S6 and the synthesis of poly-α-l-glutamate peptides using l-glutamic acid. In the present study, the crystal structure of the Escherichia coli RimK protein complexed with the ATP analogue AMP-PNP was determined at 2.05 A - resolution. Two different conformations of RimK, closed and open forms, were observed in the crystals. The structural polymorphism revealed in this study provided important information to understand the mechanism by which RimK catalyzes the synthesis of poly-α- l-glutamate peptides and the polyglutamylation of ribosomal protein S6.
AB - Bacterial RimK is an enzyme that catalyzes the polyglutamylation of the C-terminus of ribosomal protein S6 and the synthesis of poly-α-l-glutamate peptides using l-glutamic acid. In the present study, the crystal structure of the Escherichia coli RimK protein complexed with the ATP analogue AMP-PNP was determined at 2.05 A - resolution. Two different conformations of RimK, closed and open forms, were observed in the crystals. The structural polymorphism revealed in this study provided important information to understand the mechanism by which RimK catalyzes the synthesis of poly-α- l-glutamate peptides and the polyglutamylation of ribosomal protein S6.
KW - Escherichia coli
KW - Poly-α-L-glutamate synthetase
KW - RimK
KW - Structural polymorphism
UR - http://www.scopus.com/inward/record.url?scp=85049521038&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85049521038&partnerID=8YFLogxK
U2 - 10.1107/S2053230X18007689
DO - 10.1107/S2053230X18007689
M3 - Article
C2 - 29969101
AN - SCOPUS:85049521038
SN - 1744-3091
VL - 74
SP - 385
EP - 390
JO - Acta Crystallographica Section F: Structural Biology Communications
JF - Acta Crystallographica Section F: Structural Biology Communications
IS - 7
ER -