Structural property of DNA that migrates faster in gel electrophoresis, as deduced by CD spectroscopy

Akimasa Matsugami, Keiko Tani, Kiyoshi Ouhashi, Seiichi Uesugi, Mitsunori Morita, Takashi Ohyama, Masato Katahira*

*この研究の対応する著者

研究成果: Article査読

7 被引用数 (Scopus)

抄録

Bent DNAs are known to migrate slower than ordinary DNA in non-denaturing polyacrylamide gel electrophoresis. In contrast, several satellite DNAs have been shown to migrate fast. The structural property that causes the fast migration, however, is not clarified so far on molecular basis. We have investigated the structural property of a satellite DNA, which contains consecutive purine sequences and migrates faster in gel, by CD spectroscopy. Partial formation of an A-form-like structure has been suggested. Reduction in DNA length due to the formation of the A-form-like structure may be responsible for the fast migration. The pronounced rigidity of DNA may also contribute to the behavior.

本文言語English
ページ(範囲)417-425
ページ数9
ジャーナルNucleosides, Nucleotides and Nucleic Acids
25
4-6
DOI
出版ステータスPublished - 2006 6月 1
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子医療
  • 遺伝学

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