TY - JOUR
T1 - Structure and solution properties of lipidheme-microsphere
AU - Komatsu, T.
AU - Nishide, Hiroyuki
AU - Tsuchida, E.
PY - 1994
Y1 - 1994
N2 - Triglyceride microsphere emulsified with phospholipid derivative of heme (5,10,15,20-tetrakis[α,α,α,α-o-[2,2-dimethyl-20-[2-(trimethylammonioethoxy) phosphonatoxy]eicosanamido]phenyl]porphinatoiron(II); lipidheme) provides a totally synthetic artificial red cell (lipidheme-microsphere; LH-M). Its structure, solution properties and O2 binding ability are described. The particle diameter of the LH-M was ca. 90 nmφ elucidated by electron microscopy. Viscosity of the LH-M suspension (~1.5 cP) was much lower than that of human blood and the viscosity of mixed system of LH-M/human blood (1/1(v/v)) was 2.5 cP. Specific gravity, osmotic pressure, and colloid osmotic pressure of the LH-M suspension also satisfied the physiological needs. The LH-M can bind O2 reversibly in response to O2 pressure (P50(O2): 41 torr (pH 7.4, 37°C), O2 solubility of the LH-M was more than that of human blood caused by its high heme concentration.
AB - Triglyceride microsphere emulsified with phospholipid derivative of heme (5,10,15,20-tetrakis[α,α,α,α-o-[2,2-dimethyl-20-[2-(trimethylammonioethoxy) phosphonatoxy]eicosanamido]phenyl]porphinatoiron(II); lipidheme) provides a totally synthetic artificial red cell (lipidheme-microsphere; LH-M). Its structure, solution properties and O2 binding ability are described. The particle diameter of the LH-M was ca. 90 nmφ elucidated by electron microscopy. Viscosity of the LH-M suspension (~1.5 cP) was much lower than that of human blood and the viscosity of mixed system of LH-M/human blood (1/1(v/v)) was 2.5 cP. Specific gravity, osmotic pressure, and colloid osmotic pressure of the LH-M suspension also satisfied the physiological needs. The LH-M can bind O2 reversibly in response to O2 pressure (P50(O2): 41 torr (pH 7.4, 37°C), O2 solubility of the LH-M was more than that of human blood caused by its high heme concentration.
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M3 - Article
C2 - 7994409
AN - SCOPUS:0028021484
SN - 1073-1199
VL - 22
SP - 855
EP - 860
JO - Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
JF - Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
IS - 3
ER -