Substrate specificity of trna (adenine-1-)-methyltransferase from thermus thermophilus hb27

Norihiko Yamazaki, Hiroyuki Hori, Kiyoshi Ozawa, Shigeko Nakanishi, Takuya Ueda, Izumi Kumagai, Kimitsuna Watanabe, Kazuya Nishikawa

研究成果: Article査読

9 被引用数 (Scopus)


Trna (adenine-1-)-methyItransferase was purified to homogeneity from an extreme thermophile, Thermus thermophilus HB27, by several steps of column chromatographies. The molecular weight of this enzyme was about 60,000 as analyzed by SDS polyacrylamide gel electrophoresis. Km for E. coli was 100 nm and that for the methyl group donor, S-adenosyl-l-methionine, was 7.8 μm. The substrate specificity of the enzyme was investigated by using T7 RNA polymerase transcripts and tRNA fragments obtained by partial digestion with RNases. The enzyme was able to transfer the methyl group to the 3′-half fragment of E. coli initiator tRNA, however, the extent of methylation was elevated by more than five times when the 5′-half fragment was added and annealed to the 3′-half. This indicates that the main recognition site of the enzyme is within the 3′-half region of tRNA molecule, while the tertiary interaction between the T-loop and the D-loop is very effective for the adequate methylation reaction.

ジャーナルBioscience, Biotechnology and Biochemistry
出版ステータスPublished - 1994 1月

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学


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