By the synergistic catalysis of Ce4+ and Pr3+, DNA is efficiently hydrolyzed at so far the highest rate. When [Ce4+] = 10 mM and [Pr3+] = 5 mM, the half-life of dinucleotide hydrolysis at pH 7 and 50 degrees C is only 24 min. This catalytic activity is 9 times as large as that of Ce4+, which is the only prominent catalyst hitherto reported. The Ce(4+)-Nd3+ combination is also effective. These bimetallic catalytic systems are eminent tools for the future molecular biology and biotechnology.
|ジャーナル||Nucleic acids symposium series|
|出版ステータス||Published - 1995|
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