TY - JOUR
T1 - Tea catechins enhance the mRNA expression of uncoupling protein 1 in rat brown adipose tissue
AU - Nomura, Sachiko
AU - Ichinose, Takashi
AU - Jinde, Manabu
AU - Kawashima, Yu
AU - Tachiyashiki, Kaoru
AU - Imaizumi, Kazuhiko
N1 - Funding Information:
The authors are grateful to Drs. Tadashi Hase, Yoshihisa Katsuragi, Takatoshi Murase and Hideto Takase of the Kao Corporation (Tokyo, Japan) for the kind gift of TC-supplemented diets. This study was partly supported by “Establishment of Consolidated Research Institute for Advanced Science and Medical Care” (S.N. and K.I.) and a grant-in-aid from the Academic Frontier Project (2005–2010; K.I.) of the Ministry of Education, Culture, Sports, Science and Technology, Japan.
PY - 2008/12
Y1 - 2008/12
N2 - The aim of the present study was to determine whether the antiobesity effects of tea catechins (TCs) are associated with the expression of uncoupling protein 1 (UCP1) in brown adipose tissue (BAT). Male Sprague-Dawley rats were fed a high-fat (HF; 35% fat) diet for 5 weeks, then divided into four groups and fed an HF, HF with 0.5% TC (HFTC), normal-fat (NF; 5% fat) or NF with 0.5% TC (NFTC) diet for 8 weeks. At the end of the experimental period, perirenal and epididymal white adipose tissues (WATs) and interscapular BAT were isolated. The NFTC group had significantly lower perirenal WAT weights than the NF group (NF: 12.7±0.53 g; NFTC: 10.2±0.43 g; P<.01), but the HF and HFTC groups did not differ significantly. TC intake had no effects on epididymal WAT weights. The NFTC and HFTC groups had significantly lower BAT weights than the NF and HF groups, respectively. The NFTC group had significantly higher UCP1 mRNA levels in BAT than the NF group (NF: 0.35±0.02; NFTC: 0.60±0.11; P<.05), but the HF and HFTC groups did not differ significantly. Thus, TC intake in the context of the NF diet reduced perirenal WAT weight and up-regulated UCP1 mRNA expression in BAT. These results suggest that the suppressive effect of TC on body fat accumulation is associated with UCP1 expression in BAT.
AB - The aim of the present study was to determine whether the antiobesity effects of tea catechins (TCs) are associated with the expression of uncoupling protein 1 (UCP1) in brown adipose tissue (BAT). Male Sprague-Dawley rats were fed a high-fat (HF; 35% fat) diet for 5 weeks, then divided into four groups and fed an HF, HF with 0.5% TC (HFTC), normal-fat (NF; 5% fat) or NF with 0.5% TC (NFTC) diet for 8 weeks. At the end of the experimental period, perirenal and epididymal white adipose tissues (WATs) and interscapular BAT were isolated. The NFTC group had significantly lower perirenal WAT weights than the NF group (NF: 12.7±0.53 g; NFTC: 10.2±0.43 g; P<.01), but the HF and HFTC groups did not differ significantly. TC intake had no effects on epididymal WAT weights. The NFTC and HFTC groups had significantly lower BAT weights than the NF and HF groups, respectively. The NFTC group had significantly higher UCP1 mRNA levels in BAT than the NF group (NF: 0.35±0.02; NFTC: 0.60±0.11; P<.05), but the HF and HFTC groups did not differ significantly. Thus, TC intake in the context of the NF diet reduced perirenal WAT weight and up-regulated UCP1 mRNA expression in BAT. These results suggest that the suppressive effect of TC on body fat accumulation is associated with UCP1 expression in BAT.
KW - Brown adipose tissue
KW - High-fat diet
KW - Normal-fat diet
KW - Tea catechins
KW - Uncoupling protein 1
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U2 - 10.1016/j.jnutbio.2007.11.005
DO - 10.1016/j.jnutbio.2007.11.005
M3 - Article
C2 - 18479902
AN - SCOPUS:50149107253
SN - 0955-2863
VL - 19
SP - 840
EP - 847
JO - Journal of Nutritional Biochemistry
JF - Journal of Nutritional Biochemistry
IS - 12
ER -
