TY - JOUR
T1 - Temperature dependence for anammox bacteria enriched from freshwater sediments
AU - Osaka, Toshifumi
AU - Kimura, Yuya
AU - Otsubo, Yosuke
AU - Suwa, Yuichi
AU - Tsuneda, Satoshi
AU - Isaka, Kazuichi
N1 - Funding Information:
We thank Professor F. Kazama of Yamanashi University for help in sampling freshwater sediments. This study was supported by NEDO (New energy and industrial technology development organization), Japan .
Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2012/10
Y1 - 2012/10
N2 - The anoxic ammonium oxidation (anammox) process has been regarded as an attractive alternative process to treat wastewater containing high ammonium concentrations. By the implementation of anammox process at moderately low temperatures (<25°C), the anammox process will be applied to more various industrial wastewater treatments. In this study, we established enrichment cultures of anammox bacteria from freshwater sediments by using an up-flow column reactor equipped with porous polyester nonwoven fabric at moderately low temperatures. Their nitrogen conversion rates reached 0.07-0.26kg-N/m3/d. Phylogenetic analysis based on 16S rRNA gene from enrichment cultures revealed the presence of various anammox bacteria affiliated with unknown anammox bacteria as well as known anammox candidates, i.e., Candidatus Kuenenia stuttgartiensis and Candidatus Brocadia fulgida, Candidatus Scalindua wagneri. Anammox bacterial populations were influenced by enrichment conditions, i.e., seed sediments and temperature.
AB - The anoxic ammonium oxidation (anammox) process has been regarded as an attractive alternative process to treat wastewater containing high ammonium concentrations. By the implementation of anammox process at moderately low temperatures (<25°C), the anammox process will be applied to more various industrial wastewater treatments. In this study, we established enrichment cultures of anammox bacteria from freshwater sediments by using an up-flow column reactor equipped with porous polyester nonwoven fabric at moderately low temperatures. Their nitrogen conversion rates reached 0.07-0.26kg-N/m3/d. Phylogenetic analysis based on 16S rRNA gene from enrichment cultures revealed the presence of various anammox bacteria affiliated with unknown anammox bacteria as well as known anammox candidates, i.e., Candidatus Kuenenia stuttgartiensis and Candidatus Brocadia fulgida, Candidatus Scalindua wagneri. Anammox bacterial populations were influenced by enrichment conditions, i.e., seed sediments and temperature.
KW - 16S rRNA phylogenic analysis
KW - Anammox
KW - Biological nitrogen cycle
KW - Freshwater environment
KW - Moderately low temperature
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U2 - 10.1016/j.jbiosc.2012.05.003
DO - 10.1016/j.jbiosc.2012.05.003
M3 - Article
C2 - 22652085
AN - SCOPUS:84865961410
SN - 1389-1723
VL - 114
SP - 429
EP - 434
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 4
ER -