Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis

Jun Ueda; Akihito Harada; Takashi Urahama; Shinichi Machida; Kazumitsu Maehara; Masashi Hada; Yoshinori Makino; Jumpei Nogami; Naoki Horikoshi; Akihisa Osakabe; Hiroyuki Taguchi; Hiroki Tanaka; Hiroaki Tachiwana; Tatsuma Yao; Minami Yamada; Takashi Iwamoto; Ayako Isotani; Masahito Ikawa; Taro Tachibana; Yuki Okada; Hiroshi Kimura; Yasuyuki Ohkawa; Hitoshi Kurumizaka; Kazuo Yamagata

doi:10.1016/j.celrep.2016.12.065

Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis

Jun Ueda^*, Akihito Harada, Takashi Urahama, Shinichi Machida, Kazumitsu Maehara, Masashi Hada, Yoshinori Makino, Jumpei Nogami, Naoki Horikoshi, Akihisa Osakabe, Hiroyuki Taguchi, Hiroki Tanaka, Hiroaki Tachiwana, Tatsuma Yao, Minami Yamada, Takashi Iwamoto, Ayako Isotani, Masahito Ikawa, Taro Tachibana, Yuki OkadaHiroshi Kimura, Yasuyuki Ohkawa, Hitoshi Kurumizaka, Kazuo Yamagata

^*この研究の対応する著者

理工学術院総合研究所

研究成果: Article › 査読

66 被引用数 (Scopus)

抄録

Cellular differentiation is associated with dynamic chromatin remodeling in establishing a cell-type-specific epigenomic landscape. Here, we find that mouse testis-specific and replication-dependent histone H3 variant H3t is essential for very early stages of spermatogenesis. H3t gene deficiency leads to azoospermia because of the loss of haploid germ cells. When differentiating spermatogonia emerge in normal spermatogenesis, H3t appears and replaces the canonical H3 proteins. Structural and biochemical analyses reveal that H3t-containing nucleosomes are more flexible than the canonical nucleosomes. Thus, by incorporating H3t into the genome during spermatogonial differentiation, male germ cells are able to enter meiosis and beyond.

本文言語	English
ページ（範囲）	593-600
ページ数	8
ジャーナル	Cell Reports
巻	18
号	3
DOI	https://doi.org/10.1016/j.celrep.2016.12.065
出版ステータス	Published - 2017 1月 17

ASJC Scopus subject areas

生化学、遺伝学、分子生物学（全般）

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10.1016/j.celrep.2016.12.065

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Ueda, J., Harada, A., Urahama, T., Machida, S., Maehara, K., Hada, M., Makino, Y., Nogami, J., Horikoshi, N., Osakabe, A., Taguchi, H., Tanaka, H., Tachiwana, H., Yao, T., Yamada, M., Iwamoto, T., Isotani, A., Ikawa, M., Tachibana, T., ... Yamagata, K. (2017). Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis. Cell Reports, 18(3), 593-600. https://doi.org/10.1016/j.celrep.2016.12.065

Ueda, J, Harada, A, Urahama, T, Machida, S, Maehara, K, Hada, M, Makino, Y, Nogami, J, Horikoshi, N, Osakabe, A, Taguchi, H, Tanaka, H, Tachiwana, H, Yao, T, Yamada, M, Iwamoto, T, Isotani, A, Ikawa, M, Tachibana, T, Okada, Y, Kimura, H, Ohkawa, Y, Kurumizaka, H & Yamagata, K 2017, 'Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis', Cell Reports, vol. 18, no. 3, pp. 593-600. https://doi.org/10.1016/j.celrep.2016.12.065

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AU - Hada, Masashi

AU - Makino, Yoshinori

AU - Nogami, Jumpei

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AU - Tanaka, Hiroki

AU - Tachiwana, Hiroaki

AU - Yao, Tatsuma

AU - Yamada, Minami

AU - Iwamoto, Takashi

AU - Isotani, Ayako

AU - Ikawa, Masahito

AU - Tachibana, Taro

AU - Okada, Yuki

AU - Kimura, Hiroshi

AU - Ohkawa, Yasuyuki

AU - Kurumizaka, Hitoshi

AU - Yamagata, Kazuo

PY - 2017/1/17

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N2 - Cellular differentiation is associated with dynamic chromatin remodeling in establishing a cell-type-specific epigenomic landscape. Here, we find that mouse testis-specific and replication-dependent histone H3 variant H3t is essential for very early stages of spermatogenesis. H3t gene deficiency leads to azoospermia because of the loss of haploid germ cells. When differentiating spermatogonia emerge in normal spermatogenesis, H3t appears and replaces the canonical H3 proteins. Structural and biochemical analyses reveal that H3t-containing nucleosomes are more flexible than the canonical nucleosomes. Thus, by incorporating H3t into the genome during spermatogonial differentiation, male germ cells are able to enter meiosis and beyond.

AB - Cellular differentiation is associated with dynamic chromatin remodeling in establishing a cell-type-specific epigenomic landscape. Here, we find that mouse testis-specific and replication-dependent histone H3 variant H3t is essential for very early stages of spermatogenesis. H3t gene deficiency leads to azoospermia because of the loss of haploid germ cells. When differentiating spermatogonia emerge in normal spermatogenesis, H3t appears and replaces the canonical H3 proteins. Structural and biochemical analyses reveal that H3t-containing nucleosomes are more flexible than the canonical nucleosomes. Thus, by incorporating H3t into the genome during spermatogonial differentiation, male germ cells are able to enter meiosis and beyond.

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KW - testes

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Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis

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