TY - JOUR
T1 - The location of the left-handedly curved DNA sequence affects exogenous DNA expression in vivo
AU - Kamiya, Hiroyuki
AU - Fukunaga, Satoki
AU - Ohyama, Takashi
AU - Harashima, Hideyoshi
N1 - Funding Information:
This work was supported in part by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and the Japan Society for the Promotion of Science.
PY - 2007/5/1
Y1 - 2007/5/1
N2 - The intranuclear disposition of a plasmid is extremely important for transgene expression. The effects of a left-handedly curved sequence with high histone affinity on plasmid expression were examined in vivo. A naked luciferase-plasmid was delivered into mouse liver by a hydrodynamics-based injection, and the luciferase activities were quantitated at various time points. The location of the left-handedly curved sequence determined the transgene expression, without affecting the amount of intranuclear exogenous DNA. The plasmid containing the curved sequence at the location that results in the exposure of the TATA box out of the nucleosome core showed the highest expression. These results suggest that sequences with high histone affinity could control transgene expression from plasmids in vivo.
AB - The intranuclear disposition of a plasmid is extremely important for transgene expression. The effects of a left-handedly curved sequence with high histone affinity on plasmid expression were examined in vivo. A naked luciferase-plasmid was delivered into mouse liver by a hydrodynamics-based injection, and the luciferase activities were quantitated at various time points. The location of the left-handedly curved sequence determined the transgene expression, without affecting the amount of intranuclear exogenous DNA. The plasmid containing the curved sequence at the location that results in the exposure of the TATA box out of the nucleosome core showed the highest expression. These results suggest that sequences with high histone affinity could control transgene expression from plasmids in vivo.
KW - Exogenous DNA
KW - Histones
KW - Hydrodynamics-based injection
KW - Intranuclear disposition
KW - Left-handedly curved DNA
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U2 - 10.1016/j.abb.2007.02.012
DO - 10.1016/j.abb.2007.02.012
M3 - Article
C2 - 17382286
AN - SCOPUS:34247275203
SN - 0003-9861
VL - 461
SP - 7
EP - 12
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 1
ER -