抄録
We have developed the hyperprocessing technique to evaluate the stability of the cloverleaf shape of pretransfer RNA (pre-tRNA). Application of this strategy to hyperprocessible human tyrosine pre-tRNA indicated that the natural intron sequence did not contribute to stabilization of the cloverleaf shape of this pre-tRNA, while the artificial intron with elongated anticodon-stem completely inhibited hyperprocessing of it. Our data suggested that the contemporary intron sequence may be a vestige of the ancient pre-biotic world, but not has been retained as a temporal stabilizer of the pre-tRNA before the base modifications.
| 本文言語 | English |
|---|---|
| ページ（範囲） | 1782-1785 |
| ページ数 | 4 |
| ジャーナル | Bioscience, Biotechnology and Biochemistry |
| 巻 | 68 |
| 号 | 8 |
| DOI | |
| 出版ステータス | Published - 2004 8月 |
| 外部発表 | はい |
ASJC Scopus subject areas
- 食品科学
- 応用微生物学とバイオテクノロジー
- 化学（その他）
- 生化学、遺伝学、分子生物学（全般）
- 生化学
- バイオテクノロジー