We have developed the hyperprocessing technique to evaluate the stability of the cloverleaf shape of pretransfer RNA (pre-tRNA). Application of this strategy to hyperprocessible human tyrosine pre-tRNA indicated that the natural intron sequence did not contribute to stabilization of the cloverleaf shape of this pre-tRNA, while the artificial intron with elongated anticodon-stem completely inhibited hyperprocessing of it. Our data suggested that the contemporary intron sequence may be a vestige of the ancient pre-biotic world, but not has been retained as a temporal stabilizer of the pre-tRNA before the base modifications.
|ジャーナル||Bioscience, Biotechnology and Biochemistry|
|出版ステータス||Published - 2004 8月|
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