The rapid quantification and detection of nitrifying bacteria by using monoclonal antibody method

H. Ikuta*, N. Noda, Y. Ebie, A. Hirata, S. Tsuneda, M. Matsumura, Y. Inamori


研究成果: Conference article査読

4 被引用数 (Scopus)


Monoclonal antibodies against the two kinds of nitrifying bacteria Nitrosomonas europaea (IFO 14298) and Nitrobacter winogradskyi (IFO 14297) were raised and isotypes of these monoclonal antibodies, IgM and IgG1, were successfully obtained. Cross reactivities of these monoclonal antibodies against various kinds of representative heterotrophic bacteria turned out to be relatively low by competitive ELISA. In contrast, these monoclonal antibodies were very specific for nitrifying bacteria used as antigens. By means of sandwich ELISA using different isotype monoclonal antibodies such as IgM and IgG1, calibration curves were successfully developed for quantification of nitrifying bacteria. It was shown that the obtainable lower limit of quantification of N. europaea and N. winogradskyi were 7.0 × 106 N/ml and were 6.0 × 105 N/ml, respectively. Nitrifying bacteria in activated sludge of advanced domestic wastewater treatment johkaso were counted by sandwich ELISA and MPN methods. The bacterial number estimated by MPN method was lower than that estimated by sandwich ELISA. It was indicated that this monoclonal antibody method could be used as a quick and powerful tool for estimating and controlling the population of nitrifying bacteria in the advanced domestic wastewater treatment processes.

ジャーナルWater Science and Technology
出版ステータスPublished - 2000
イベントWater Quality Management in Asia (Asian Waterqual'99) - Taipei, Taiwan
継続期間: 1999 10月 181999 10月 20

ASJC Scopus subject areas

  • 環境工学
  • 水の科学と技術


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