The RNA-binding protein HuD regulates neuronal cell identity and maturation

Wado Akamatsu, Hiroaki Fujihara, Takayuki Mitsuhashi, Masato Yano, Shinsuke Shibata, Yoshika Hayakawa, Hirotaka James Okano, Shin Ichi Sakakibara, Hiroshi Takano, Toshiya Takano, Takao Takahashi, Tetsuo Noda, Hideyuki Okano*


研究成果: Article査読

174 被引用数 (Scopus)


Neural Hu proteins (HuB/C/D) are RNA-binding proteins that have been shown to induce neuronal differentiation activity when overexpressed in immature neural progenitor cells or undifferentiated neuronal tumors. Newly generated HuD-deficient mice exhibited a transient impaired-cranial-nerve-development phenotype at an early embryonic stage. Adult HuD-deficient mice exhibited an abnormal hind-limb reflex and poor rotarod performance. Analysis of neurosphere formation revealed that the number and self-renewal capacity of the neural stem/progenitor cells were increased in HuD-deficient mice. HuD-deficient primary neurospheres also generated a smaller number of neurons. Cohort analysis of the cellular proliferative activity by using BrdUrd and iododeoxuridine labeling revealed that the number of differentiating quiescent cells in the embryonic cerebral wall was decreased. Long-term administration of BrdUrd revealed that the number of slowly dividing stem cells in the adult subventricular zone was increased in the HuD-deficient mice. Taken together, the results suggest that HuD is required at multiple points during neuronal development, including negative regulation of proliferative activity and neuronal cell-fate acquisition of neural stem/progenitor cells.

ジャーナルProceedings of the National Academy of Sciences of the United States of America
出版ステータスPublished - 2005 3月 22

ASJC Scopus subject areas

  • 一般


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