TY - JOUR
T1 - Thyrotropin-Releasing Hormone (TRH) Is the Major Prolactin-Releasing Factor in the Bullfrog Hypothalamus
AU - Nakajima, Keisuke
AU - Uchida, Daisuke
AU - Sakai, Makoto
AU - Takahashi, Noriyuki
AU - Yanagisawa, Tadashi
AU - Yamamoto, Kazutoshi
AU - Kikuyama, Sakae
PY - 1993/1
Y1 - 1993/1
N2 - A substance exhibiting potent activity in stimulating the release of prolactin from bullfrog (Rana catesbeiana) pituitary in vitro was isolated from an acid extract of bullfrog hypothalami by gel-filtration chromatography (Sephadex G-15), ion-exchange chromatography (Mono-S HR 5/5), and reverse-phase high-performance liquid chromatography (TSK-gel ODS-120T). Its amino acid composition was similar to that of synthetic thyrotropin-releasing hormone (TRH). Radioimmunoassay confirmed that the substance had TRH immunoreactivity. Moreover, it exhibited the same chromatographic behavior as that of synthetic TRH. These results clearly indicate that the isolated hypothalamic substance is TRH, and that it is the major prolactin-releasing factor present in the bullfrog hypothalamus.
AB - A substance exhibiting potent activity in stimulating the release of prolactin from bullfrog (Rana catesbeiana) pituitary in vitro was isolated from an acid extract of bullfrog hypothalami by gel-filtration chromatography (Sephadex G-15), ion-exchange chromatography (Mono-S HR 5/5), and reverse-phase high-performance liquid chromatography (TSK-gel ODS-120T). Its amino acid composition was similar to that of synthetic thyrotropin-releasing hormone (TRH). Radioimmunoassay confirmed that the substance had TRH immunoreactivity. Moreover, it exhibited the same chromatographic behavior as that of synthetic TRH. These results clearly indicate that the isolated hypothalamic substance is TRH, and that it is the major prolactin-releasing factor present in the bullfrog hypothalamus.
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U2 - 10.1006/gcen.1993.1004
DO - 10.1006/gcen.1993.1004
M3 - Article
C2 - 8428643
AN - SCOPUS:0027434248
SN - 0016-6480
VL - 89
SP - 11
EP - 16
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
IS - 1
ER -