TY - JOUR
T1 - Time-lapse single-cell transcriptomics reveals modulation of histone H3 for dormancy breaking in fission yeast
AU - Tsuyuzaki, Hayato
AU - Hosokawa, Masahito
AU - Arikawa, Koji
AU - Yoda, Takuya
AU - Okada, Naoyuki
AU - Takeyama, Haruko
AU - Sato, Masamitsu
N1 - Funding Information:
We thank Taro Nakamura and Chikashi Shimoda for advice on fission yeast spore biology, Peter Thorpe for advice on spore experiments, Sachiyo Aburatani and Hisashi Anbutsu for support in OIL, Toru Maruyama for advice on informatics and Kiyofumi Takahashi and Chikako Sakanashi for technical support in RNA-seq. This study was supported by JSPS KAKENHI JP25291041, JP15H01359, JP16H04787, JP16H01317 and JP18K19347 to M.S. This study was also supported by The Uehara Memorial Foundation and by Waseda University grants for Special Research Projects 2017B-242, 2017B-243, 2018B-222 and 2019C-570 to M.S. This work was partially supported by the JSPS Core-to-Core Program, A. Advanced Research Networks and by the Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from AMED under Grant Number JP19am0101104.
Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - How quiescent cells break dormancy is a key issue in eukaryotic cells including cancer. Fungal spores, for example, remain quiescent for long periods until nourished, although the mechanisms by which dormancy is broken remain enigmatic. Transcriptome analysis could provide a clue, but methods to synchronously germinate large numbers of spores are lacking, and thus it remains a challenge to analyse gene expression upon germination. Hence, we develop methods to assemble transcriptomes from individual, asynchronous spore cells of fission yeast undergoing germination to assess transcriptomic changes over time. The virtual time-lapse analyses highlights one of three copies of histone H3 genes whose transcription fluctuates during the initial stage of germination. Disruption of this temporal fluctuation causes defects in spore germination despite no visible defects in other stages of the life cycle. We conclude that modulation of histone H3 expression is a crucial ‘wake-up’ trigger at dormancy breaking.
AB - How quiescent cells break dormancy is a key issue in eukaryotic cells including cancer. Fungal spores, for example, remain quiescent for long periods until nourished, although the mechanisms by which dormancy is broken remain enigmatic. Transcriptome analysis could provide a clue, but methods to synchronously germinate large numbers of spores are lacking, and thus it remains a challenge to analyse gene expression upon germination. Hence, we develop methods to assemble transcriptomes from individual, asynchronous spore cells of fission yeast undergoing germination to assess transcriptomic changes over time. The virtual time-lapse analyses highlights one of three copies of histone H3 genes whose transcription fluctuates during the initial stage of germination. Disruption of this temporal fluctuation causes defects in spore germination despite no visible defects in other stages of the life cycle. We conclude that modulation of histone H3 expression is a crucial ‘wake-up’ trigger at dormancy breaking.
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U2 - 10.1038/s41467-020-15060-y
DO - 10.1038/s41467-020-15060-y
M3 - Article
C2 - 32152323
AN - SCOPUS:85081608405
SN - 2041-1723
VL - 11
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 1265
ER -