@article{2a8690739e864b00b8a678008d8093b1,
title = "Transcription factor Spi-B-dependent and-independent pathways for the development of Peyer's patch M cells",
abstract = "Although many of the biological features of microfold cells (M cells) have been known for many years, the molecular mechanisms of M-cell development and antigen recognition have remained unclear. Here, we report that Umod is a novel M-cell-specific gene, the translation products of which might contribute to the uptake function of M cells. Transcription factor Spi-B was also specifically expressed in M cells among non-hematopoietic lineages. Spi-B-deficient mice showed reduced expression of most, but not all, other M-cell-specific genes and M-cell surface markers. Whereas uptake of Salmonella Typhimurium via M cells was obviously reduced in Spi-B-deficient mice, the abundance of intratissue cohabiting bacteria was comparable between wild-type and Spi-B-deficient mice. These data indicate that there is a small M-cell population with developmental regulation that is Spi-B independent; however, Spi-B is probably a candidate master regulator of M-cell functional maturation and development by another pathway.",
author = "S. Sato and S. Kaneto and N. Shibata and Y. Takahashi and H. Okura and Y. Yuki and J. Kunisawa and H. Kiyono",
note = "Funding Information: We thank Lee-Ann Garret-Sinha (The State University of New York at Buffalo, Buffalo, NY) and M. Celeste Simon (University of Pennsylvania School of Medicine, Philadelphia, PA) for providing the Spib−/− mice, Anson W. Lowe (Stanford University, Palo Alto, CA) for providing the Gp2−/− mice, and Steven E. Domino (University of Michigan, Ann Arbor, MI) for providing the Fut1lacZ/lacZ mice. We also thank Hidenori Matsui (Kitasato University, Tokyo, Japan) for the S. Typhimurium; Hiroshi Sagara (The University of Tokyo, Tokyo, Japan) for electron microscopy; and Yoshihito Minoda, Jun Yoshida, and Ai Yamashita for technical assistance. Our work is supported by grants from: the Ministry of Education, Culture, Sports, Science, and Technology of Japan (Grant-in-Aid for challenging Exploratory Research (23659199 to S.S.) and for Scientific Research S (23229004 to H.K.)); the Global Center of Excellence Program of the Center of Education and Research for Advanced Genome-based Medicine (to H.K.); and the Core Research for Evolutional Science and Technology Program of the Japan Science and Technology Agency (to H.K.).",
year = "2013",
month = jul,
doi = "10.1038/mi.2012.122",
language = "English",
volume = "6",
pages = "838--846",
journal = "Mucosal Immunology",
issn = "1933-0219",
publisher = "Nature Publishing Group",
number = "4",
}