@article{1053c69a049049d4b61708d666bf343f,
title = "Transcription Factors as Evolvable Biosensors",
abstract = "One of the most prominent features of genetically encoded biosensors (GEBs) is their evolvability—the ability to invent new sensory functions using mutations. Among the GEBs, the transcription factor-based biosensors (TF-biosensors) is the focus of this review. We also discuss how this class of sensors can be highly evolvable and how we can exploit it. With an established platform for directed evolution, researchers can create, or evolve, new TF-biosensors. Directed evolution experiments have revealed the TF-biosensors{\textquoteright} evolvability, which is based partially on their characteristic physicochemical properties.",
keywords = "Genetic switches, biosensors",
author = "Daisuke Umeno and Yuki Kimura and Shigeko Kawai-Noma",
note = "Funding Information: This work was partially supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology [JSPS Kakenhi Grants 15H04189, 15K14228, 16H 06450]. D.U. expresses the most profound appreciation to Professor Mizuo Maeda (RIKEN, Japan) for scientific guidance over the years. Publisher Copyright: 2021 {\textcopyright} The Japan Society for Analytical Chemistry",
year = "2021",
month = may,
doi = "10.2116/analsci.20SCR12",
language = "English",
volume = "37",
pages = "699--705",
journal = "Analytical Sciences",
issn = "0910-6340",
publisher = "Japan Society for Analytical Chemistry",
number = "5",
}