TY - JOUR
T1 - Transfection microarrays for high-throughput phenotypic screening of genes involved in cell migration.
AU - Onuki-Nagasaki, Reiko
AU - Nagasaki, Akira
AU - Hakamada, Kazumi
AU - Uyeda, Taro Q.P.
AU - Fujita, Satoshi
AU - Miyake, Masato
AU - Miyake, Jun
PY - 2010
Y1 - 2010
N2 - Cell migration is important in several biological phenomena, such as cancer metastasis. Therefore, the identification of genes involved in cell migration might facilitate the discovery of antimetastatic drugs. However, screening of genes by the current methods can be complicated by factors related to cell stimulation, for example, abolition of contact inhibition and the release inflammatory cytokines from wounded cells during examinations of wound healing in vitro. To overcome these problems and identify genes involved in cell migration, in this chapter we describe the use of transfection microarrays for high-throughput phenotypic screening.
AB - Cell migration is important in several biological phenomena, such as cancer metastasis. Therefore, the identification of genes involved in cell migration might facilitate the discovery of antimetastatic drugs. However, screening of genes by the current methods can be complicated by factors related to cell stimulation, for example, abolition of contact inhibition and the release inflammatory cytokines from wounded cells during examinations of wound healing in vitro. To overcome these problems and identify genes involved in cell migration, in this chapter we describe the use of transfection microarrays for high-throughput phenotypic screening.
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U2 - 10.1007/978-1-60761-657-3_13
DO - 10.1007/978-1-60761-657-3_13
M3 - Article
C2 - 20387151
AN - SCOPUS:77954064742
SN - 1064-3745
VL - 629
SP - 193
EP - 203
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -