Transition of β-actinin isoforms during development of chicken skeletal muscle

Yoko Asami, Takashi Funatsu, Shin'ichi Ishiwata*


    研究成果: Article査読

    2 被引用数 (Scopus)


    We examined by means of the immunoblotting technique the transition of β-actinin isoforms during the development of the chicken from 5 day embryo to adult. As an antigen, β-actinin was prepared from adult chicken breast muscle (pectoralis major) and polyclonal antibody was obtained by injecting undenatured β-actinin into a rabbit. Immunoblotting examination of breast muscle at several stages of development (except 5 day embryo, in which the whole body minus the head and limbs was examined) showed that the species of β-actinin subunits change during development: 1) βI is already present in 5 day embryo, whereas βII appears only after 9 days. 2) In 5 day embryo, we found, instead of βII, a new subunit (designated βIII) that cross-reacts with the antibody, has the apparent molecular weight of 30,000 daltons and has a slightly alkaline isoelectric point compared with βI. The content of βIII gradually decreased and βIII completely disappeared a week after hatching. Such a type of transition of the isoforms in β-actinin subunits is similar to that observed in other muscle proteins. The transition of β-actinin isoforms may correlate to the organization of an I-Z-I brush, especially to the length determination of thin filaments, because the developmental stage at which βIII disappears coincides with that at which the length of thin filaments is strictly determined.

    ジャーナルJournal of Biochemistry
    出版ステータスPublished - 1988 1月

    ASJC Scopus subject areas

    • 統計学、確率および不確実性
    • 応用数学
    • 生理学(医学)
    • 放射線学、核医学およびイメージング
    • 分子生物学
    • 生化学


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