TY - JOUR
T1 - Trehangelins A, B and C, novel photo-oxidative hemolysis inhibitors produced by an endophytic actinomycete, Polymorphospora rubra K07-0510
AU - Nakashima, Takuji
AU - Okuyama, Ryuki
AU - Kamiya, Yoshiyuki
AU - Matsumoto, Atsuko
AU - Iwatsuki, Masato
AU - Inahashi, Yuki
AU - Yamaji, Kenzaburo
AU - Takahashi, Yoko
AU - Omura, Satoshi
N1 - Funding Information:
This work was supported, in part, by funds from Quality Assurance Flamework of Higher Education from the Ministry of Education, Culture, Sports, Sciences and Technology (MEXT), Japan. We are grateful to Mr Toru Kimura in our laboratory for his technical assistance, Ms Noriko Sato of Kitasato University for measurements of NMR spectra and Dr Tomoyasu Hirose for advice on the structure.
PY - 2013/6
Y1 - 2013/6
N2 - Three new natural products, designated trehangelins A, B and C, were isolated by solvent extraction, silica gel and octadecylsilyl silica gel column chromatographies and subsequent preparative HPLC from the cultured broth of an endophytic actinomycete strain, Polymorphospora rubra K07-0510. The trehangelins consisted of a trehalose moiety and two angelic acid moieties. Trehangelins A (IC 50 value, 0.1 mg ml -1) and C (IC 50 value, 0.4 mg ml -1), with symmetric structures, showed potent inhibitory activity against hemolysis of red blood cells induced by light-activated pheophorbide a. However, trehangelin B, with an asymmetric structure, displayed only a slight inhibition (IC 50 value, 1.0 mg ml -1).
AB - Three new natural products, designated trehangelins A, B and C, were isolated by solvent extraction, silica gel and octadecylsilyl silica gel column chromatographies and subsequent preparative HPLC from the cultured broth of an endophytic actinomycete strain, Polymorphospora rubra K07-0510. The trehangelins consisted of a trehalose moiety and two angelic acid moieties. Trehangelins A (IC 50 value, 0.1 mg ml -1) and C (IC 50 value, 0.4 mg ml -1), with symmetric structures, showed potent inhibitory activity against hemolysis of red blood cells induced by light-activated pheophorbide a. However, trehangelin B, with an asymmetric structure, displayed only a slight inhibition (IC 50 value, 1.0 mg ml -1).
KW - Polymorphospora rubra
KW - chemical screening
KW - endophytic actinomycete
KW - hemolysis inhibitor
KW - trehangelins
UR - http://www.scopus.com/inward/record.url?scp=84879641548&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84879641548&partnerID=8YFLogxK
U2 - 10.1038/ja.2013.17
DO - 10.1038/ja.2013.17
M3 - Article
C2 - 23591606
AN - SCOPUS:84879641548
SN - 0021-8820
VL - 66
SP - 311
EP - 317
JO - Journal of Antibiotics
JF - Journal of Antibiotics
IS - 6
ER -