Two KaiA-binding domains of cyanobacterial circadian clock protein KaiC

Yasuhito Taniguchi; Akihiro Yamaguchi; Atsushi Hijikata; Hideo Iwasaki; Kyoko Kamagata; Masahiro Ishiura; Mitiko Go; Takao Kondo

doi:10.1016/S0014-5793(01)02408-5

Two KaiA-binding domains of cyanobacterial circadian clock protein KaiC

Yasuhito Taniguchi, Akihiro Yamaguchi, Atsushi Hijikata, Hideo Iwasaki, Kyoko Kamagata, Masahiro Ishiura, Mitiko Go, Takao Kondo^*

^*この研究の対応する著者

研究成果: Article › 査読

54 被引用数 (Scopus)

抄録

kaiABC, a gene cluster, encodes KaiA, KaiB and KaiC proteins that are essential to circadian rhythms in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942. Kai proteins can interact with each other in all possible combinations. This study identified two KaiA-binding domains (C_KABD1 and C_KABD2) in KaiC at corresponding regions of its duplicated structure. Clock mutations on the two domains and kaiA altered the strength of C_KABD-KaiA interactions assayed by the yeast two-hybrid system. Thus, interaction between KaiA and KaiC through C_KABD1 and C_KABD2 is likely important for circadian timing in the cyanobacterium.

本文言語	English
ページ（範囲）	86-90
ページ数	5
ジャーナル	FEBS Letters
巻	496
号	2-3
DOI	https://doi.org/10.1016/S0014-5793(01)02408-5
出版ステータス	Published - 2001 5月 11
外部発表	はい

ASJC Scopus subject areas

生物理学
構造生物学
生化学
分子生物学
遺伝学
細胞生物学

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10.1016/S0014-5793(01)02408-5

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title = "Two KaiA-binding domains of cyanobacterial circadian clock protein KaiC",

abstract = "kaiABC, a gene cluster, encodes KaiA, KaiB and KaiC proteins that are essential to circadian rhythms in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942. Kai proteins can interact with each other in all possible combinations. This study identified two KaiA-binding domains (CKABD1 and CKABD2) in KaiC at corresponding regions of its duplicated structure. Clock mutations on the two domains and kaiA altered the strength of CKABD-KaiA interactions assayed by the yeast two-hybrid system. Thus, interaction between KaiA and KaiC through CKABD1 and CKABD2 is likely important for circadian timing in the cyanobacterium.",

keywords = "Circadian rhythm, Cyanobacterium, Protein-protein interaction, Synechococcus, kai gene",

author = "Yasuhito Taniguchi and Akihiro Yamaguchi and Atsushi Hijikata and Hideo Iwasaki and Kyoko Kamagata and Masahiro Ishiura and Mitiko Go and Takao Kondo",

note = "Funding Information: This research was supported by grants from the Japanese Ministry of Education, Science and Culture (11233203, 11440234, 11558089, 11694199 to T.K. and M.I. and 12207002, 12208006, 1559007, 11480189 to M.G.).",

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AU - Taniguchi, Yasuhito

AU - Yamaguchi, Akihiro

AU - Hijikata, Atsushi

AU - Iwasaki, Hideo

AU - Kamagata, Kyoko

AU - Ishiura, Masahiro

AU - Go, Mitiko

AU - Kondo, Takao

N1 - Funding Information: This research was supported by grants from the Japanese Ministry of Education, Science and Culture (11233203, 11440234, 11558089, 11694199 to T.K. and M.I. and 12207002, 12208006, 1559007, 11480189 to M.G.).

PY - 2001/5/11

Y1 - 2001/5/11

N2 - kaiABC, a gene cluster, encodes KaiA, KaiB and KaiC proteins that are essential to circadian rhythms in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942. Kai proteins can interact with each other in all possible combinations. This study identified two KaiA-binding domains (CKABD1 and CKABD2) in KaiC at corresponding regions of its duplicated structure. Clock mutations on the two domains and kaiA altered the strength of CKABD-KaiA interactions assayed by the yeast two-hybrid system. Thus, interaction between KaiA and KaiC through CKABD1 and CKABD2 is likely important for circadian timing in the cyanobacterium.

AB - kaiABC, a gene cluster, encodes KaiA, KaiB and KaiC proteins that are essential to circadian rhythms in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942. Kai proteins can interact with each other in all possible combinations. This study identified two KaiA-binding domains (CKABD1 and CKABD2) in KaiC at corresponding regions of its duplicated structure. Clock mutations on the two domains and kaiA altered the strength of CKABD-KaiA interactions assayed by the yeast two-hybrid system. Thus, interaction between KaiA and KaiC through CKABD1 and CKABD2 is likely important for circadian timing in the cyanobacterium.

KW - Circadian rhythm

KW - Cyanobacterium

KW - Protein-protein interaction

KW - Synechococcus

KW - kai gene

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AN - SCOPUS:0035843996

SN - 0014-5793

VL - 496

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EP - 90

JO - FEBS Letters

JF - FEBS Letters

IS - 2-3

ER -

Two KaiA-binding domains of cyanobacterial circadian clock protein KaiC

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