TY - GEN
T1 - Ultimate hydrogel thermal-transition based flow control system for user-friendly particle and cell sorting
AU - Sugino, H.
AU - Ozaki, K.
AU - Shirasaki, Y.
AU - Aoki, T.
AU - Arakawa, T.
AU - Yoon, D. H.
AU - Shoji, S.
AU - Funatsu, T.
PY - 2010
Y1 - 2010
N2 - This paper reports an ultimate hydrogel-based flow control system for fluorescently-labeled bioparticle sorting. We previously developed a hydrogel-based sorter. But the system requires the mixture of bio-sample and hydrogel to perform flow switching. This might be a concern when sorted samples are analyzed by sensitive analytical techniques. To overcome this issue, we developed a new system which doesn't require the mixing of sample and hydrogel. With the system, we successfully sorted microspheres and bacterial cells without mixing. The system is practical for following sample analysis because there is no need to separate sample from hydrogel after sorting.
AB - This paper reports an ultimate hydrogel-based flow control system for fluorescently-labeled bioparticle sorting. We previously developed a hydrogel-based sorter. But the system requires the mixture of bio-sample and hydrogel to perform flow switching. This might be a concern when sorted samples are analyzed by sensitive analytical techniques. To overcome this issue, we developed a new system which doesn't require the mixing of sample and hydrogel. With the system, we successfully sorted microspheres and bacterial cells without mixing. The system is practical for following sample analysis because there is no need to separate sample from hydrogel after sorting.
KW - Cell
KW - Fluorescence-activated cell sorter
KW - Sorting
KW - Thermoreversible gelation polymer
UR - http://www.scopus.com/inward/record.url?scp=84884332837&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884332837&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84884332837
SN - 9781618390622
T3 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
SP - 244
EP - 246
BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Y2 - 3 October 2010 through 7 October 2010
ER -