Uniform and high throughput agarose gel micro droplet generation device for single cell analysis

T. Hirose*, Y. Hoshino, D. H. Yoon, A. Nakahara, T. Mori, T. Sekiguchi, H. Takeyama, S. Shoji

*この研究の対応する著者

研究成果: Conference contribution

1 被引用数 (Scopus)

抄録

We designed and fabricated high throughput agarose gel micro droplet (GMD) generation device for single cell function analysis. Temperature control of each elements of the device is important issue because of the thermo-oriented sol-gel transfer of agarose gel. Uniform GMD of 50 μm in diameter which is suitable size for cell culturing media is achieved with size deviation less than 1.58% and generation rate of about 190deoplets/sec. Single E.coli cell encapsulation and cultivation for 5 hours are successfully achieved in an agarose GMD.

本文言語English
ホスト出版物のタイトルProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
出版社Chemical and Biological Microsystems Society
ページ500-502
ページ数3
ISBN(印刷版)9780979806452
出版ステータスPublished - 2012 1月 1
イベント16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
継続期間: 2012 10月 282012 11月 1

出版物シリーズ

名前Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Conference

Conference16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
国/地域Japan
CityOkinawa
Period12/10/2812/11/1

ASJC Scopus subject areas

  • 化学工学(その他)
  • バイオエンジニアリング

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