TY - GEN
T1 - Use of real-time PCR to examine the relationship between ammonia oxidizing bacterial populations and nitrogen removal efficiency in a small decentralized treatment system 'Johkasou'
AU - Nakagawa, G.
AU - Ebie, Y.
AU - Tsuneda, S.
AU - Matsumura, M.
AU - Inamori, Y.
PY - 2007
Y1 - 2007
N2 - The aim of this study was to examine the relationship between ammonia oxidizing bacterial populations and biological nitrogen removal in a small on-site domestic wastewater treatment system "Johkasou". The population dynamics of ammonia oxidizing bacteria (AOB) in six full-scale advanced Johkasous was surveyed using real-time PCR assay over a period of one year. These Johkasous were selected to compare the AOB populations in different treatment performance. When the effluent NH4-N concentration was higher than 2 mg L-1, it was difficult to meet the effluent standard of advanced Johkasous (T-N 10 mg L-1). In contrast, the nitrogen removal efficiency was hardly affected by nitrite oxidation and denitrification in these systems. In other words, ammonia oxidation was a rate-limiting step. Furthermore, we focused on the relationship between NH4-N loading per AOB cell and nitrogen removal. Real time PCR monitoring results demonstrated that it is important to regulate NH4-N loading per AOB cell below 210 pg cell-1 day-1 to meet the effluent standard of advanced Johkasou. It is considered that NH4-N loading per AOB cell is a useful parameter for determining suitable nitrogen loading and small decentralized system design.
AB - The aim of this study was to examine the relationship between ammonia oxidizing bacterial populations and biological nitrogen removal in a small on-site domestic wastewater treatment system "Johkasou". The population dynamics of ammonia oxidizing bacteria (AOB) in six full-scale advanced Johkasous was surveyed using real-time PCR assay over a period of one year. These Johkasous were selected to compare the AOB populations in different treatment performance. When the effluent NH4-N concentration was higher than 2 mg L-1, it was difficult to meet the effluent standard of advanced Johkasous (T-N 10 mg L-1). In contrast, the nitrogen removal efficiency was hardly affected by nitrite oxidation and denitrification in these systems. In other words, ammonia oxidation was a rate-limiting step. Furthermore, we focused on the relationship between NH4-N loading per AOB cell and nitrogen removal. Real time PCR monitoring results demonstrated that it is important to regulate NH4-N loading per AOB cell below 210 pg cell-1 day-1 to meet the effluent standard of advanced Johkasou. It is considered that NH4-N loading per AOB cell is a useful parameter for determining suitable nitrogen loading and small decentralized system design.
KW - Ammonia oxidizing bacteria
KW - Biological nitrogen removal
KW - Domestic wastewater
KW - Johkasou
KW - Real-time PCR
KW - Small decentralized system
UR - http://www.scopus.com/inward/record.url?scp=34248580761&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34248580761&partnerID=8YFLogxK
U2 - 10.2166/wst.2007.146
DO - 10.2166/wst.2007.146
M3 - Conference contribution
C2 - 17506439
AN - SCOPUS:34248580761
SN - 1843395827
SN - 9781843395829
T3 - Water Science and Technology
SP - 203
EP - 210
BT - Small Water and Wastewater Systems VII
A2 - Martinez, S.G.
ER -