@article{245889d9c7ff44e9b574ab0c264e06ba,
title = "Variable domain antibodies specific for viral hemorrhagic septicemia virus (VHSV) selected from a randomized IgNAR phage display library",
abstract = "Phage display libraries are used to screen for nucleotide sequences that encode immunoglobulin variable (V) regions that are specific for a target antigen. We previously constructed an immunoglobulin new antigen receptor (IgNAR) phage display library. Here we used this library to obtain an IgNAR V region that is specific for viral hemorrhagic septicemia virus (VHSV). A phage clone (clone 653) was found to be specific for VHSV by the biopanning method. The V region of clone 653 was used to construct a 6 × His tagged recombinant IgNAR-653 V protein (rIgNAR-653) using the Escherichia coli pET system. The rIgNAR-653 protein bound specifically to VHSV, confirming its activity.",
keywords = "IgNAR, Neutralization activity, Phage display library, VHSV",
author = "Maki Ohtani and Hikima, {Jun ichi} and Jung, {Tae Sung} and Hidehiro Kondo and Ikuo Hirono and Haruko Takeyama and Takashi Aoki",
note = "Funding Information: This work was supported by Grant-in-Aid for Scientific research [KAKENHI KIBAN KENKYU (A) 21248025 ] from the Ministry of Education, Culture, Sports, Science and Technology, Japan . This research was also supported by a grant from the World Class University Program ( R32-10253 ), funded by the Korean Ministry of Education, Science, and Technology of South Korea .",
year = "2013",
month = feb,
doi = "10.1016/j.fsi.2012.11.041",
language = "English",
volume = "34",
pages = "724--728",
journal = "Fish and Shellfish Immunology",
issn = "1050-4648",
publisher = "Academic Press Inc.",
number = "2",
}